Induction of insulin resistance by high‐sucrose feeding does not raise mean arterial blood pressure but impairs haemodynamic responses to insulin in rats
- 1 September 2002
- journal article
- Published by Wiley in British Journal of Pharmacology
- Vol. 137 (2), 185-196
- https://doi.org/10.1038/sj.bjp.0704864
Abstract
1. This study was undertaken to further investigate the effects of a sucrose-enriched diet on vascular function and insulin sensitivity in rats. 2. Male Sprague-Dawley rats were randomized to receive a sucrose- or regular rat chow-diet for 4 weeks. A first group of sucrose- and chow-fed rats was instrumented with pulsed Doppler flow probes and intravascular catheters to determine blood pressure, heart rate, regional blood flows and insulin sensitivity in conscious rats. Insulin sensitivity was assessed by the euglycemic hyperinsulinemic clamp technique. Glucose transport activity was examined in isolated muscles by using the glucose analogue [(3)H]-2-deoxy-D-glucose. A second group of sucrose- and chow-fed rats was used to obtain information regarding nitric oxide synthase (NOS) isozymes protein expression in muscles, and determine endothelin content in vascular tissues isolated from both dietary groups. 3. Sucrose feeding was found to induce insulin resistance, but had no effect on resting blood pressure, heart rate, or regional haemodynamics. This insulin resistance was accompanied by alteration in the vascular responses to insulin. Insulin-mediated skeletal muscle vasodilation was impaired, whereas the mesenteric vasoconstrictor response was potentiated in sucrose-fed rats. A reduction in eNOS protein content in muscle and an increase in vascular endothelin peptide were noted in these animals. Moreover, a reduction in insulin-simulated glucose transport activity was also noted in muscles isolated from sucrose-fed rats. 4. Together these data suggest that a cluster of metabolic and haemodynamic abnormalities occur in response to the intake of simple sugars in rats.Keywords
This publication has 49 references indexed in Scilit:
- Review of Alterations in Endothelial Nitric Oxide Production in DiabetesHypertension, 1998
- Insulin-stimulated production of nitric oxide is inhibited by wortmannin. Direct measurement in vascular endothelial cells.JCI Insight, 1996
- Endothelial-Type Nitric Oxide Synthase (ec-NOS) in Skeletal Muscle Fibers: Mitochondrial RelationshipsBiochemical and Biophysical Research Communications, 1995
- Insulin resistance, diabetes, and the insulin-regulated trafficking of GLUT-4.The Journal of cell biology, 1994
- Differential effects of hyperinsulinemia and carbohydrate metabolism on sympathetic nerve activity and muscle blood flow in humans.JCI Insight, 1993
- Skeletal muscle blood flow. A possible link between insulin resistance and blood pressure.Hypertension, 1993
- Intra-arterial pressure alterations during tail-cuff blood pressure measurements in normotensive and hypertensive ratsJournal Of Hypertension, 1990
- Somatostatin inhibition of fructose-induced hypertension.Hypertension, 1989
- Fructose-induced insulin resistance and hypertension in rats.Hypertension, 1987
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970