A Fluorescent Chemosensor for Sodium Based on Photoinduced Electron Transfer
- 24 December 2002
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 75 (3), 549-555
- https://doi.org/10.1021/ac0205107
Abstract
A new optical sensor suitable for practical measurement of sodium in serum and whole blood samples is described. The optical sensor is based on a novel PET (photoinduced eletron transfer) fluoroionophore immobilized in a hydrophilic polymer layer. The design concept of the fluoroionophore follows the receptor-spacer-fluorophore approach to sensor design using intramolecular PET-based signal transduction. Key to the development of this sensor is the identification of a nitrogen-containing, sodium-binding ionophore, coupled with a fluorophore having the correct spectral and electron-accepting properties. The slope of the sensor is ∼0.5%/mM in the typical clinically significant range of 120−160 mM. This sensor has been implemented into a disposable cartridge, used for a commercially available critical care analyzer (Roche OPTI CCA) with precision better than ±1 mM (1 SD). The sensor displays excellent stability against hydrolysis and oxidation, leading to slope changes <5% after 9 months wet storage at 30 °C. On the basis of this design concept, fluoroionophores for other cations such as potassium, calcium and magnesium can be prepared by substitution of the ionophore.Keywords
This publication has 11 references indexed in Scilit:
- Fluorescent Sensors for Zn2+Based on a Fluorescein Platform: Synthesis, Properties and Intracellular DistributionJournal of the American Chemical Society, 2001
- Novel Alkali Cation Chemosensors Based on N-9-Anthrylaza-crown EthersOrganic Letters, 2001
- Design principles of fluorescent molecular sensors for cation recognitionCoordination Chemistry Reviews, 2000
- Fluorescence-based ion sensing using potential-sensitive dyesSensors and Actuators B: Chemical, 1995
- New Fluorescent Model Compounds for the Study of Photoinduced Electron Transfer: The Influence of a Molecular Electric Field in the Excited StateAngewandte Chemie-International Edition, 1995
- Molecular fluorescent signalling with ‘fluor–spacer–receptor’ systems: approaches to sensing and switching devices via supramolecular photophysicsChemical Society Reviews, 1992
- Photo-induced electron transfer as a general design logic for fluorescent molecular sensors for cationsBiosensors, 1989
- Optical sensors: An ion-selective optrode for potassiumAnalytica Chimica Acta, 1987
- Methyl transfers. 10. The Marcus equation application to soft nucleophilesJournal of the American Chemical Society, 1985
- A Novel Colorimetric Reagent for Potassium Based on Crown Ether Complex FormationAnalytical Letters, 1977