Engineering of Human Cartilage Rods: Potential Application for Penile Prostheses
- 1 October 2002
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Urology
- Vol. 168 (4), 1794-1797
- https://doi.org/10.1016/s0022-5347(05)64415-1
Abstract
Natural penile prostheses created from the patient’s own cells may eliminate the biocompatibility risks associated with artificial prostheses. We previously demonstrated that autologous cartilaginous rods could be created in animal corpus cavernosum as penile prostheses in situ by transplanting autologous chondrocytes on biodegradable polymer scaffolds. In the present study we investigated the possibility of engineering human cartilage rods for potential use as penile prostheses. Chondrocytes isolated from human ear were seeded on rod shaped biodegradable polymer scaffolds (1.2 cm. in diameter, 6.0 cm. long). The seeded scaffolds were maintained in stirred bioreactors for 1 month. Subsequently, the seeded scaffolds were implanted subcutaneously into athymic rats. The specimens were retrieved 2 months after implantation, and histological, structural and mechanical properties were analyzed. The mechanical properties of the engineered prostheses were compared to those of silicone prostheses. Human chondrocytes seeded onto polymer scaffolds formed milk-white cartilaginous rods of the same size as the initial implants. Histological analyses using hematoxylin and eosin, toluidine blue and alcian blue showed mature and well-formed chrondrocytes in the retrieved implants. The engineered human cartilaginous rods were flexible, elastic and able to withstand high degrees of compressive forces. The mechanical properties were comparable to those of commercially available silicone prostheses. Transplantation of chrondrocytes isolated from human ear on polymer scaffolds resulted in the formation of human cartilage rods with the appropriate mechanical properties required for use as penile prostheses. This study demonstrates the feasibility of creating human cartilage rods with a large dimension. This technology may be useful for patients who need penile reconstruction.Keywords
This publication has 8 references indexed in Scilit:
- AUTOLOGOUS ENGINEERED CARTILAGE RODS FOR PENILE RECONSTRUCTIONJournal of Urology, 1999
- CARTILAGE RODS AS A POTENTIAL MATERIAL FOR PENILE RECONSTRUCTIONJournal of Urology, 1998
- Complications and Reimplantation of Penile ImplantsInternational Journal of Urology, 1997
- Penile Gangrene: A Complication of Penile ProsthesisScandinavian Journal of Urology and Nephrology, 1995
- Endoscopic Treatment of Vesicoureteral Reflux with a Chondrocyte-Alginate SuspensionJournal of Urology, 1994
- Injectable Alginate Seeded with Chondrocytes as a Potential Treatment for Vesicoureteral RefluxJournal of Urology, 1993
- Small-Carrion penile prosthesisUrology, 1975
- PhalloplastyJournal of Urology, 1952