Evaluation of the quality of blood components prepared using the Reveos automated blood processing system
- 28 May 2013
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 105 (3), 225-235
- https://doi.org/10.1111/vox.12051
Abstract
The Reveos automated blood processing system has been developed to combine primary and secondary processing of whole-blood units, resulting in a plasma unit, a red-blood-cell concentrate and an interim platelet unit per input. The aim of this study was to determine product specifications and in vitro quality of components produced by the Reveos system. Whole blood was processed using the Reveos system and compared with historical Reference units produced using semi-automated methods. Reveos red cells were leucoreduced and stored in SAGM at 4°C. Reveos plasma was frozen at -30°C and factor activity was assessed after thawing. Reference red cell, plasma and buffy coats were produced by top and bottom processing. Leucoreduced Reveos and Reference platelet concentrates were prepared by pooling four interim platelet units or four buffy coats, respectively, with SSP+. Processing with the Reveos system was faster (76 min) than semi-automated separation (92 min). The red cell and platelet yields were higher in the units prepared by the Reveos system. The Reference and Reveos red cell and plasma units had very similar in vitro quality parameters. The platelet concentrates were also similar in many in vitro parameters, including pH, glucose and lactate metabolism, hypotonic shock response and phosphatidylserine expression, although platelet activation markers (CD62P and cytokine levels) were higher in the Reveos units. The Reveos system can improve blood component efficiencies through reductions in processing time, whilst maintaining similar component quality.Keywords
This publication has 27 references indexed in Scilit:
- PAS‐G supports platelet reconstitution after cryopreservation in the absence of plasmaTransfusion, 2013
- Evaluation of the quality of blood components obtained after automated separation of whole blood by a new multiunit processorTransfusion, 2012
- Automated processing of whole blood units: operational value and in vitro quality of final blood components2012
- Evaluation of the automated collection and extended storage of apheresis platelets in additive solutionTransfusion, 2011
- The effect of pathogen reduction technology (Mirasol) on platelet quality when treated in additive solution with low plasma carryoverVox Sanguinis, 2011
- Cryopreservation of buffy-coat-derived platelet concentrates in dimethyl sulfoxide and platelet additive solutionCryobiology, 2011
- Storage of interim platelet units for 18 to 24 hours before pooling: in vitro studyTransfusion, 2010
- Buffy‐coat platelet variables and metabolism during storage in additive solutions or plasmaTransfusion, 2008
- Evaluation of platelet activation and cytokine release during storage of platelet concentrates processed from buffy coats either manually or by the automated OrbiSac systemTransfusion, 2007
- Fully automated processing of buffy-coat-derived pooled platelet concentratesTransfusion, 2004