Current Status and Advances in Quantitative Proteomic Mass Spectrometry
Open Access
- 6 March 2013
- journal article
- review article
- Published by Hindawi Limited in International Journal of Proteomics
- Vol. 2013, 1-12
- https://doi.org/10.1155/2013/180605
Abstract
The accurate quantitation of proteins and peptides in complex biological systems is one of the most challenging areas of proteomics. Mass spectrometry-based approaches have forged significant in-roads allowing accurate and sensitive quantitation and the ability to multiplex vastly complex samples through the application of robust bioinformatic tools. These relative and absolute quantitative measures using label-free, tags, or stable isotope labelling have their own strengths and limitations. The continuous development of these methods is vital for increasing reproducibility in the rapidly expanding application of quantitative proteomics in biomarker discovery and validation. This paper provides a critical overview of the primary mass spectrometry-based quantitative approaches and the current status of quantitative proteomics in biomedical research.Keywords
This publication has 94 references indexed in Scilit:
- Accurate Quantification of Cardiovascular Biomarkers in Serum Using Protein Standard Absolute Quantification (PSAQ™) and Selected Reaction MonitoringMolecular & Cellular Proteomics, 2012
- More than 100,000 Detectable Peptide Species Elute in Single Shotgun Proteomics Runs but the Majority is Inaccessible to Data-Dependent LC−MS/MSJournal of Proteome Research, 2011
- Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasmaNature Biotechnology, 2009
- Targeted proteomic strategy for clinical biomarker discoveryMolecular Oncology, 2008
- MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantificationNature Biotechnology, 2008
- Selected reaction monitoring for quantitative proteomics: a tutorialMolecular Systems Biology, 2008
- Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networksProceedings of the National Academy of Sciences, 2007
- Protein biomarker discovery and validation: the long and uncertain path to clinical utilityNature Biotechnology, 2006
- Multiplexed protein measurement: technologies and applications of protein and antibody arraysNature Reviews Drug Discovery, 2006
- Mass spectrometry-based proteomicsNature, 2003