Phylogeny and Characterization of Three nifH -Homologous Genes from Paenibacillus azotofixans
Open Access
- 1 June 2003
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (6), 3658-3662
- https://doi.org/10.1128/aem.69.6.3658-3662.2003
Abstract
In this paper, we report the cloning and characterization of three Paenibacillus azotofixans DNA regions containing genes involved in nitrogen fixation. Sequencing analysis revealed the presence of nifB1H1D1K1 gene organization in the 4,607-bp Sac I DNA fragment. This is the first report of linkage of a nifB open reading frame upstream of the structural nif genes. The second ( nifB2H2 ) and third ( nifH3 ) nif homologues are confined within the 6,350-bp Hin dIII and 2,840-bp Eco RI DNA fragments, respectively. Phylogenetic analysis demonstrated that NifH1 and NifH2 form a monophyletic group among cyanobacterial NifH proteins. NifH3, on the other hand, clusters among NifH proteins of the highly divergent methanogenic archaea.Keywords
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