Interaction of C-reactive protein with artificial phosphatidylcholine bilayers
- 1 September 1979
- journal article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 281 (5727), 155-157
- https://doi.org/10.1038/281155a0
Abstract
C-Reactive protein (CRP), the most characteristic of the 'acute phase proteins' (ref. 1) is thought to participate in the mediation and/or modulation of acute inflammatory processes, but its exact function is unknown. CRP has a Ca2+-dependent binding specificity for phosphorylcholine, the polar head group of two widely distributed lipids, lecithin (phosphatidylcholine, PC) and sphingomyelin (SM). A number of observations suggest that at least some of the biological activities of CRP depend on its interaction with phospholipids of cell membranes. In addition, interaction of CRP with PC- and SM-containing lipid dispersions and with PC-containing liposomes can activate the complement system. We report here that binding of CRP to model membranes of PC requires the incorporation into the bilayer of lysophosphatidylcholine (LPC). Thus, a disturbance of the molecular organisation of the bilayer appears to be necessary for binding of CRP. These findings provide a possible biochemical explanation for binding of CRP to damaged but not intact cell membranes and might be relevant to its biological function.Keywords
This publication has 18 references indexed in Scilit:
- Control of the Acute Phase ResponseJCI Insight, 1978
- Effect of small molecules on the dipalmitoyl lecithin liposomal bilayer: III. Phase transition in lipid bilayerThe Journal of Membrane Biology, 1977
- Phospholipid head-group conformations; intermolecular interactions and cholesterol effectsBiochemistry, 1977
- Delipidation of the phosphatidylcholine exchange protein from beef liver by detergentsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1975
- Solubilization of membranes by detergentsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1975
- Barrier properties of lecithin/lysolecithin mixturesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- Action of pure phospholipase A2 and phospholipase C on human erythrocytes and ghostsBiochimica et Biophysica Acta (BBA) - Biomembranes, 1971
- Phosphatidylcholine vesicles. Formation and physical characteristicsBiochemistry, 1969
- C-reactive Protein - A Phagocytosis-promoting FactorScandinavian Journal of Clinical and Laboratory Investigation, 1969
- STUDIES OF ACUTE PHASE PROTEINThe Journal of Experimental Medicine, 1961