Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli.
- 1 January 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (1), 201-204
- https://doi.org/10.1073/pnas.77.1.201
Abstract
The complete nucleotide sequence of the 23S RNA gene from the rnnB operon of E. coli was determined. The sequences of both strands of the entire gene were determined, most of the sequence was independently confirmed using alternate restriction fragments, and all restriction cuts overlapped. The DNA region corresponding to mature 23S rRNA contains 2904 nucleotides. Kethoxal-reactive sites protected by 30S subunits are found between positions 2300-2800, placing the subunit interface in this region of the molecule. The functional importance of this region is further supported by studies by other investigators, including homology with chloroplast and mitochondrial rRNA.This publication has 31 references indexed in Scilit:
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