Fertility Results after Vaginal Deposition of Frozen‐Thawed Buck Semen Diluted with Two Different Extenders Using One‐ or Two‐Step Procedures
- 18 January 2011
- journal article
- research article
- Published by Wiley in Reproduction in Domestic Animals
- Vol. 46 (1), 82-86
- https://doi.org/10.1111/j.1439-0531.2010.01586.x
Abstract
In two field trials (T1 and T2), the effect of two different extenders for buck semen was tested. Semen from six (T1) and seven (T2) bucks of the Norwegian Dairy Goat breed was diluted either in a milk-based extender containing egg yolk (M) or in a commercially available extender without egg yolk [Andromed(®) (A)]. Dilution in M was performed in a two-step procedure including centrifugation of the ejaculates and removal of the supernatant, while dilution in A was performed in one step. During the two trials (T1 and T2) 514 and 714, does, respectively, were artificially inseminated during natural oestrus, and the farmers performed the inseminations themselves after attending an artificial insemination (AI) training course. Vaginal insemination with 200 × 10(6) spermatozoa diluted in M resulted in a 25-day non-return rate (NRR) and kidding rate of 37.3% and 24.5%, respectively, while semen diluted in A resulted in 31.7% NRR and a kidding rate of 19.8% (T1). In T2, NRR and kidding rate for AI performed with semen diluted with M were 42.7% and 28.5%, respectively, while dilution in A gave 37.2% NRR and a kidding rate of 26.8%. There was no significant effect of extender in the two trials [T1:p=0.068 (NRR), p=0.148 (kidding rate), T2:p=0.096 (NRR), p=0.38 (kidding rate)], but farmer had a significant effect on the fertility parameters in both trials. In conclusion, the present studies may indicate that Andromed(®) is suitable for buck semen. However, more research is necessary to confirm the results and to improve the fertility of does after vaginal AI with frozen-thawed semen.Keywords
This publication has 11 references indexed in Scilit:
- Effect of cervical and vaginal insemination with liquid semen stored at room temperature on fertility of goatsAnimal Reproduction Science, 2005
- Successful pregnancies with directional freezing of large volume buck semenTheriogenology, 2005
- Fertility results after different thawing procedures for ram semen frozen in minitubes and mini strawsTheriogenology, 2004
- Effect of milk- and TRIS-based extenders on the fertility of sheep inseminated vaginally once or twice with liquid semenTheriogenology, 2003
- Effect of deposition site and sperm number on the fertility of sheep inseminated with liquid semenVeterinary Record, 2002
- Production and storage of goat semen for artificial inseminationAnimal Reproduction Science, 2000
- Comparison of bacteriological qualities of various egg yolk sources and the in vitro and in vivo fertilizing potential of bovine semen frozen in egg yolk or lecithin based diluentsTheriogenology, 1998
- Foetal loss in dairy goats: An epidemiological study in 22 herdsSmall Ruminant Research, 1998
- Artificial Insemination with Frozen Semen in Ewes at Different Times of the Breeding SeasonReproduction in Domestic Animals, 1991
- Egg Yolk-Coagulating Enzyme in the Semen and Cowper's Gland of the GoatNature, 1957