Isolation and characterisation of cell wall polymers from the heavily lignified tissues of olive (Olea europaea) seed hull
- 31 December 1995
- journal article
- Published by Elsevier BV in Carbohydrate Polymers
- Vol. 27 (4), 285-294
- https://doi.org/10.1016/0144-8617(95)00068-2
Abstract
Cell wall material (CWM) was prepared from olive seed hull, which is heavily lignified and very tough. The material was cryomilled and delignified with chlorite/acetic acid for 9 h to give the holocellulose. Polymers were solubilised from the holocellulose by sequential extraction with cyclohexane-trans-1,2-diamine-NNN'N'-tetra-acetate (CDTA, Na salt), DMSO, 0.5, 1 and 4 m KOH and 4 m KOH + borate to leave the α-cellulose residue. The suspension of α-cellulose on neutralisation released a small amount of pectic material virtually free of xylan to give α'-cellulose. The polymers from the various extracts were fractionated by graded precipitation with ethanol prior to anion-exchange chromatography, and selected fractions were subjected to methylation analysis. During delignification, glucuronoxylans with relatively low degrees of polymerisation (DP) and xylan-pectic polysaccharide complexes linked to degraded lignin were solubilised. A proportion of the xylan-pectic polysaccharide complexes were solubilised by 0.5 m KOH. The major hemicellulosic polysaccharides of the olive seed hulls are glucuronoxylans, which occur as highly branched short chains, with DP of 30–60; or slightly branched chains with DP of 90–110. Partial acid hydrolysis of the major acidic xylan, gel-filtration chromatography and methylation analysis allowed us to propose a tentative structure for the major glucuronoxylan in which one residue of GlcpA occurs in each 14 continuously linked Xylp residues in a regular structure.Keywords
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