Ethylene Signaling Negatively Regulates Freezing Tolerance by Repressing Expression of CBF and Type-A ARR Genes in Arabidopsis
Top Cited Papers
- 15 June 2012
- journal article
- Published by Oxford University Press (OUP) in THE PLANT CELL ONLINE
- Vol. 24 (6), 2578-2595
- https://doi.org/10.1105/tpc.112.098640
Abstract
The phytohormone ethylene regulates multiple aspects of plant growth and development and responses to environmental stress. However, the exact role of ethylene in freezing stress remains unclear. Here, we report that ethylene negatively regulates plant responses to freezing stress in Arabidopsis thaliana. Freezing tolerance was decreased in ethylene overproducer1 and by the application of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid but increased by the addition of the ethylene biosynthesis inhibitor aminoethoxyvinyl glycine or the perception antagonist Ag+. Furthermore, ethylene-insensitive mutants, including etr1-1, ein4-1, ein2-5, ein3-1, and ein3 eil1, displayed enhanced freezing tolerance. By contrast, the constitutive ethylene response mutant ctr1-1 and EIN3-overexpressing plants exhibited reduced freezing tolerance. Genetic and biochemical analyses revealed that EIN3 negatively regulates the expression of CBFs and type-A Arabidopsis response regulator5 (ARR5), ARR7, and ARR15 by binding to specific elements in their promoters. Overexpression of these ARR genes enhanced the freezing tolerance of plants. Thus, our study demonstrates that ethylene negatively regulates cold signaling at least partially through the direct transcriptional control of cold-regulated CBFs and type-A ARR genes by EIN3. Our study also provides evidence that type-A ARRs function as key nodes to integrate ethylene and cytokinin signaling in regulation of plant responses to environmental stress.Keywords
This publication has 78 references indexed in Scilit:
- Direct transcriptional control of the Arabidopsis immune receptor FLS2 by the ethylene-dependent transcription factors EIN3 and EIL1Proceedings of the National Academy of Sciences of the United States of America, 2010
- A Subset of Cytokinin Two-component Signaling System Plays a Role in Cold Temperature Stress Response in ArabidopsisJournal of Biological Chemistry, 2010
- EIN3/EIL1 cooperate with PIF1 to prevent photo-oxidation and to promote greening of Arabidopsis seedlingsProceedings of the National Academy of Sciences of the United States of America, 2009
- Cytokinin and auxin interaction in root stem-cell specification during early embryogenesisNature, 2008
- The negative regulator of plant cold responses, HOS1, is a RING E3 ligase that mediates the ubiquitination and degradation of ICE1Proceedings of the National Academy of Sciences of the United States of America, 2006
- The response regulator 2 mediates ethylene signalling and hormone signal integration in ArabidopsisThe EMBO Journal, 2004
- Plant Responses to Ethylene Gas Are Mediated by SCFEBF1/EBF2-Dependent Proteolysis of EIN3 Transcription FactorCell, 2003
- EIN3-Dependent Regulation of Plant Ethylene Hormone Signaling by Two Arabidopsis F Box Proteins: EBF1 and EBF2Cell, 2003
- Five components of the ethylene-response pathway identified in a screen for weak ethylene-insensitive mutants in ArabidopsisProceedings of the National Academy of Sciences of the United States of America, 2003
- CTR1, a negative regulator of the ethylene response pathway in arabidopsis, encodes a member of the Raf family of protein kinasesCell, 1993