Rice potassium transporter OsHAK1 is essential for maintaining potassium‐mediated growth and functions in salt tolerance over low and high potassium concentration ranges
- 5 June 2015
- journal article
- research article
- Published by Wiley in Plant, Cell & Environment
- Vol. 38 (12), 2747-2765
- https://doi.org/10.1111/pce.12585
Abstract
Potassium (K) absorption and translocation in plants rely upon multiple K transporters for adapting varied K supply and saline conditions. Here, we report the expression patterns and physiological roles of OsHAK1, a member belonging to the KT/KUP/HAK gene family in rice (Oryza sativa L.). The expression of OsHAK1 is up‐regulated by K deficiency or salt stress in various tissues, particularly in the root and shoot apical meristem, the epidermises and steles of root, and vascular bundles of shoot. Both oshak1 knockout mutants in comparison to their respective Dongjin or Manan wild types showed a dramatic reduction in K concentration and stunted root and shoot growth. Knockout of OsHAK1 reduced the K absorption rate of unit root surface area by ∼50–55 and ∼30%, and total K uptake by ∼80 and ∼65% at 0.05–0.1 and 1 mm K supply level, respectively. The root net high‐affinity K uptake of oshak1 mutants was sensitive to salt stress but not to ammonium supply. Overexpression of OsHAK1 in rice increased K uptake and K/Na ratio. The positive relationship between K concentration and shoot biomass in the mutants suggests that OsHAK1 plays an essential role in K‐mediated rice growth and salt tolerance over low and high K concentration ranges.Keywords
Funding Information
- National Natural Science Foundation (31361140357)
- National R&D Program for Transgenic Crops
- Fundamental Research Funds for the Central Universities (KYTZ201404)
- 111 Project (12009)
- Innovative Research Team Development Plan of the Ministry of Education of China
- PAPD of Jiangsu Higher Education Institutions Project
This publication has 72 references indexed in Scilit:
- Knockdown of a Rice Stelar Nitrate Transporter Alters Long-Distance Translocation But Not Root InfluxPlant Physiology, 2012
- K+ Transport by the OsHKT2;4 Transporter from Rice with Atypical Na+ Transport Properties and Competition in Permeation of K+ over Mg2+ and Ca2+ IonsPlant Physiology, 2011
- High-Affinity K+ Transport in Arabidopsis: AtHAK5 and AKT1 Are Vital for Seedling Establishment and Postgermination Growth under Low-Potassium ConditionsPlant Physiology, 2010
- Competition between uptake of ammonium and potassium in barley and Arabidopsis roots: molecular mechanisms and physiological consequencesJournal of Experimental Botany, 2010
- HKT transporter-mediated salinity resistance mechanisms in Arabidopsis and monocot crop plantsTrends in Plant Science, 2009
- Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different functions and kinetic properties in uptake and translocationThe Plant Journal, 2009
- KT/HAK/KUP potassium transporters gene family and their whole-life cycle expression profile in rice (Oryza sativa)Molecular Genetics and Genomics, 2008
- The Ionic Environment Controls the Contribution of the Barley HvHAK1 Transporter to Potassium AcquisitionPlant Physiology, 2008
- Plant KT/KUP/HAK Potassium Transporters: Single Family - Multiple FunctionsAnnals of Botany, 2007
- Regulation of K+ absorption in plant root cells by external K+: interplay of different plasma membrane K+ transportersJournal of Experimental Botany, 1997