Linking Crystallographic Model and Data Quality
Top Cited Papers
- 25 May 2012
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 336 (6084), 1030-1033
- https://doi.org/10.1126/science.1218231
Abstract
In macromolecular x-ray crystallography, refinement R values measure the agreement between observed and calculated data. Analogously, Rmerge values reporting on the agreement between multiple measurements of a given reflection are used to assess data quality. Here, we show that despite their widespread use, Rmerge values are poorly suited for determining the high-resolution limit and that current standard protocols discard much useful data. We introduce a statistic that estimates the correlation of an observed data set with the underlying (not measurable) true signal; this quantity, CC*, provides a single statistically valid guide for deciding which data are useful. CC* also can be used to assess model and data quality on the same scale, and this reveals when data quality is limiting model improvement.Keywords
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