Ubiquinone biosynthesis Cloning of the genes coding for chorismate pyruvate‐lyase and 4‐hydroxybenzoate octaprenyl transferase from Escherichia coli
- 3 August 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 307 (3), 347-350
- https://doi.org/10.1016/0014-5793(92)80710-x
Abstract
Chorismate pyruvate-lyase activity was detected in extracts of Escherichia coli. 4-Hydroxybenzoate was identified as the product of the enzymatic reaction by chemical derivatization and GC-MS analysis. The ubiC gene, coding for the chorismate pyruvate-lyase, was cloned and sequenced. The molecular weight of the gene product was calculated as 18.776 Da and confirmed by expression of the protein in E. coli minicells. The ubiA gene, coding for the 4-hydroxybenzoate octaprenyl transferase, was identified by sequence homology and complementation of a ubiA− strain. It is located directly downstream of ubiC in a typical operon structureKeywords
This publication has 16 references indexed in Scilit:
- COQ2 is a candidate for the structural gene encoding para-hydroxybenzoate:polyprenyltransferase.Journal of Biological Chemistry, 1992
- λ ZAP: a bacteriophage λ expression vector within vivoexcision propertiesNucleic Acids Research, 1988
- The DNA sequences encoding plsB and dgk loci of Escherichia coli.Journal of Biological Chemistry, 1983
- Biosynthesis of UbiquinoneVitamins & Hormones, 1983
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences of the United States of America, 1977
- Role of quinones in electron transport to oxygen and nitrate in Escherichia coli. Studies with a ubiA− menA− double quinone mutantBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1977
- Preliminary studies on the isolation and metabolism of an intermediate in aromatic biosynthesis: chorismic acidBiochemical Journal, 1964