Implantation in Vivo and Retrieval of Artificial Structures Consisting of Rabbit and Human Urothelium and Human Bladder Muscle
- 1 August 1993
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Urology
- Vol. 150 (2 Part 2), 608-612
- https://doi.org/10.1016/s0022-5347(17)35561-1
Abstract
The harvest of human bladder muscle and urothelial cells with subsequent growth may be useful for tissue replacement in genitourinary reconstruction. We previously reported the development of a system for the harvest, delivery and growth of rabbit urothelium in vivo using biodegradable polymers. We have now expanded and adapted this system for the harvest and in vivo implantation of human bladder urothelial and muscle cells. Synthetic polymer fibers of polyglycolic acid can serve as a scaffold and a delivery vehicle for the implantation of rabbit uroepithelial cells into athymic host animals. The polymers, which slowly degrade in vivo, allow the urothelial cells to survive at the implant site. We demonstrate that polyglycolic acid polymers support the proliferation of rabbit urothelial cells in situ and can serve as a maleable substrate for the creation of new urological structures that replace the degrading polymer fibers. We also show that human urothelial cells and bladder muscle cells, when implanted on polyglycolic acid fibers, from new urological structures in vivo composed of both cell types. The human cell-polymer xenografts can be recovered from host animals at extended times after implantation. These data suggest that feasibility of using polyglycolic acid polymers as substrates for the creation of human urothelial and muscle grafts for genitourinary reconstruction.Keywords
This publication has 21 references indexed in Scilit:
- Synthetic Polymers Seeded with Chondrocytes Provide a Template for New Cartilage FormationPlastic and Reconstructive Surgery, 1991
- Hepatocyte culture on biodegradable polymeric substratesBiotechnology & Bioengineering, 1991
- Immune Responses to Allogeneic and Xenogeneic Implants of Collagen and Collagen DerivativesClinical Orthopaedics and Related Research, 1990
- New Methods of Drug DeliveryScience, 1990
- Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures.The Journal of cell biology, 1988
- Increased production of collagenin Vivo by hepatocytes and nonparenchymal cells in rats with carbon tetrachloride-induced hepatic fibrosisHepatology, 1988
- Selective cell transplantation using bioabsorbable artificial polymers as matricesJournal of Pediatric Surgery, 1988
- Monoclonal Antibody to 5-Bromo- and 5-Iododeoxyuridine: A New Reagent for Detection of DNA ReplicationScience, 1982
- Successful Use of a Physiologically Acceptable Artificial Skin in the Treatment of Extensive Burn InjuryAnnals of Surgery, 1981
- GRAFTING OF BURNS WITH CULTURED EPITHELIUM PREPARED FROM AUTOLOGOUS EPIDERMAL CELLSThe Lancet, 1981