Enterococcal Biofilm Formation and Virulence in an Optimized Murine Model of Foreign Body-Associated Urinary Tract Infections
- 1 October 2010
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 78 (10), 4166-4175
- https://doi.org/10.1128/iai.00711-10
Abstract
Catheter-associated urinary tract infections (CAUTIs) constitute the majority of nosocomial UTIs and pose significant clinical challenges. Enterococcal species are among the predominant causative agents of CAUTIs. However, very little is known about the pathophysiology of Enterococcus -mediated UTIs. We optimized a murine model of foreign body-associated UTI in order to mimic conditions of indwelling catheters in patients. In this model, the presence of a foreign body elicits major histological changes and induces the expression of several proinflammatory cytokines in the bladder. In addition, in contrast to naïve mice, infection of catheter-implanted mice with Enterococcus faecalis induced the specific expression of interleukin 1β (IL-1β) and macrophage inflammatory protein 1α (MIP-1α) in the bladder. These responses resulted in a favorable niche for the development of persistent E. faecalis infections in the murine bladders and kidneys. Furthermore, biofilm formation on the catheter implant in vivo correlated with persistent infections. However, the enterococcal autolytic factors GelE and Atn (also known as AtlA), which are important in biofilm formation in vitro , are dispensable in vivo . In contrast, the housekeeping sortase A (SrtA) is critical for biofilm formation and virulence in CAUTIs. Overall, this murine model represents a significant advance in the understanding of CAUTIs and underscores the importance of urinary catheterization during E. faecalis uropathogenesis. This model is also a valuable tool for the identification of virulence determinants that can serve as potential antimicrobial targets for the treatment of enterococcal infections.Keywords
This publication has 82 references indexed in Scilit:
- Contribution of Autolysin and Sortase A during Enterococcus faecalis DNA-Dependent Biofilm DevelopmentInfection and Immunity, 2009
- A murine model of urinary tract infectionNature Protocols, 2009
- A family of fibrinogen-binding MSCRAMMs from Enterococcus faecalisMicrobiology, 2009
- A fratricidal mechanism is responsible for eDNA release and contributes to biofilm development of Enterococcus faecalisMolecular Microbiology, 2009
- G-CSF induction early in uropathogenicEscherichia coliinfection of the urinary tract modulates host immunityCellular Microbiology, 2008
- Streptozocin-Induced Diabetic Mouse Model of Urinary Tract InfectionInfection and Immunity, 2008
- Regulation of Autolysis-Dependent Extracellular DNA Release by Enterococcus faecalis Extracellular Proteases Influences Biofilm DevelopmentJournal of Bacteriology, 2008
- Development and Use of an Efficient System for Random mariner Transposon Mutagenesis To Identify Novel Genetic Determinants of Biofilm Formation in the Core Enterococcus faecalis GenomeApplied and Environmental Microbiology, 2008
- Relative Contributions of Enterococcus faecalis OG1RF Sortase-Encoding Genes, srtA and bps ( srtC ), to Biofilm Formation and a Murine Model of Urinary Tract InfectionInfection and Immunity, 2007
- Importance of theebp(Endocarditis‐ and Biofilm‐Associated Pilus) Locus in the Pathogenesis ofEnterococcus faecalisAscending Urinary Tract InfectionThe Journal of Infectious Diseases, 2007