Free Space Iron Pools in Roots

Abstract
A rapid and simple method for the determination of a ferric Fe pool in the free space of roots is described. Formation of this pool depended on the source Fe in the nutrient solution. During growth in water culture at pH 5-6 with Fe-EDTA, a free space pool of 500-1000 nmol Fe per gram fresh weight was formed in the roots of bean (Phaseolus vulgaris L. var. Prelude), maize (Zea mays L. var. Capella), and chlorophytum (Chlorophytum comosum [Thumb.] Jacques). No significant pool (less than 100 nmol per gram fresh weight) was formed with ferrioxamine. Upon impending Fe deficiency, bean and chlorophytum were able to mobilize this pool. Fe-deficient bean plants mobilized Fe from the free space Fepool of another plant in the same vessel.