Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient
Open Access
- 2 October 2012
- journal article
- Published by Optica Publishing Group in Biomedical Optics Express
- Vol. 3 (11), 2707-2719
- https://doi.org/10.1364/BOE.3.002707
Abstract
The scattering anisotropy, g, of tissue can be a powerful metric of tissue structure, and is most directly measured via goniometry and fitting to the Henyey-Greenstein phase function. We present a method based on an independent attenuation measurement of the scattering coefficient along with Monte Carlo simulations to account for multiple scattering, allowing the accurate determination of measurement of g for tissues of thickness within the quasi-ballistic regime. Simulations incorporating the experimental geometry and bulk optical properties show that significant errors occur in extraction of g values, even for tissues of thickness less than one scattering length without modeling corrections. Experimental validation is provided by determination of g in mouse muscle tissues and it is shown that the obtained values are independent of thickness. In addition we present a simple deconvolution-based method and show that it provides excellent estimates for high anisotropy values (above 0.95) when coupled with an independent attenuation measurement.Keywords
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