Acaricide Treatment Affects Viral Dynamics in Varroa destructor-Infested Honey Bee Colonies via both Host Physiology and Mite Control
Open Access
- 1 January 2012
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 78 (1), 227-235
- https://doi.org/10.1128/aem.06094-11
Abstract
Honey bee ( Apis mellifera ) colonies are declining, and a number of stressors have been identified that affect, alone or in combination, the health of honey bees. The ectoparasitic mite Varroa destructor , honey bee viruses that are often closely associated with the mite, and pesticides used to control the mite population form a complex system of stressors that may affect honey bee health in different ways. During an acaricide treatment using Apistan (plastic strips coated with tau-fluvalinate), we analyzed the infection dynamics of deformed wing virus (DWV), sacbrood virus (SBV), and black queen cell virus (BQCV) in adult bees, mite-infested pupae, their associated Varroa mites, and uninfested pupae, comparing these to similar samples from untreated control colonies. Titers of DWV increased initially with the onset of the acaricide application and then slightly decreased progressively coinciding with the removal of the Varroa mite infestation. This initial increase in DWV titers suggests a physiological effect of tau-fluvalinate on the host's susceptibility to viral infection. DWV titers in adult bees and uninfested pupae remained higher in treated colonies than in untreated colonies. The titers of SBV and BQCV did not show any direct relationship with mite infestation and showed a variety of possible effects of the acaricide treatment. The results indicate that other factors besides Varroa mite infestation may be important to the development and maintenance of damaging DWV titers in colonies. Possible biochemical explanations for the observed synergistic effects between tau-fluvalinate and virus infections are discussed.Keywords
This publication has 54 references indexed in Scilit:
- Emerging and re-emerging viruses of the honey bee (Apis melliferaL.)Veterinary Research, 2010
- Interactions between Nosema microspores and a neonicotinoid weaken honeybees (Apis mellifera)Environmental Microbiology, 2010
- Deformed Wing Virus Implicated in Overwintering Honeybee Colony LossesApplied and Environmental Microbiology, 2009
- The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR ExperimentsClinical Chemistry, 2009
- Economic valuation of the vulnerability of world agriculture confronted with pollinator declineEcological Economics, 2009
- Modulation of influenza virus replication by alteration of sodium ion transport and protein kinase C activityAntiviral Research, 2008
- Validation of reference genes for gene expression studies in the honey bee,Apis mellifera, by quantitative real-time RT-PCRApidologie, 2008
- Varroosis – the Ongoing Crisis in Bee KeepingJournal für Verbraucherschutz und Lebensmittelsicherheit, 2008
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Development of early infestations by the miteVarroa jacobsoni in honey-bee (Apis mellifera) colonies in cold climatesExperimental and Applied Acarology, 1991