Central Nervous System Activities of Hypericum origanifolium Extract via GABAergic and Opioidergic Mechanisms
- 18 August 2012
- journal article
- research article
- Published by Wiley in Phytotherapy Research
- Vol. 27 (6), 877-884
- https://doi.org/10.1002/ptr.4801
Abstract
Pharmacological effects of hydroalcoholic extract prepared from Hypericum origanifolium Willd. (Guttiferae) on behavioral parameters and pain perceptions of mice were investigated in this study. Depression, anxiety, spontaneous locomotor activity, and motor coordination parameters of mice were assessed by modified forced swimming, hole board, activity cage, and rota‐rod tests, respectively. In addition, antinociceptive effect was evaluated by performing hot‐plate, tail‐clip, and formalin tests. Reboxetine (20 mg/kg), diazepam (1 mg/kg), and morphine (10 mg/kg) were used as reference antidepressant, anxiolytic, and analgesic drugs, respectively. Phytochemical analyses exhibited that chlorogenic acid (2317.12 ppm) and rutin (2108.79 ppm) were the main phenolic compounds in the H. origanifolium extract. The extract (50, 100, and 250 mg/kg) induced significant antidepressant, anxiolytic, and antinociceptive activities following the acute administrations. Anxiolytic effect was antagonized by flumazenil (a benzodiazepine receptor antagonist, 2.5 mg/kg, i.p.) pre‐treatment, which indicated the participation of GABA(A)‐benzodiazepine receptor complex in the activity. Moreover, centrally and peripherally mediated antinociception reversed by naloxone (a non‐selective opioid receptor antagonist, 5 mg/kg, i.p.) pre‐treatment, indicating the involvement of opioid system in the pharmacological action. These findings are the first to indicate the pharmacological effects of the H. origanifolium extract on the emotional state and pain perceptions of mice. Copyright © 2012 John Wiley & Sons, Ltd.Keywords
This publication has 43 references indexed in Scilit:
- Synthesis and analgesic activity of some acetamide derivativesJournal of Enzyme Inhibition and Medicinal Chemistry, 2011
- New antidepressant drug candidate:Hypericum montbrettiextractNatural Product Research, 2011
- The Anti-Immobility Effect of Hyperoside on the Forced Swimming Test in Rats is Mediated by the D2-Like Receptors ActivationPlanta Medica, 2010
- Anxiolytic‐like effects of standardized extract of Justicia pectoralis (SEJP) in mice: Involvement of GABA/benzodiazepine in receptorPhytotherapy Research, 2010
- Antinociceptive Properties of the Hydroalcoholic Extract and the Flavonoid Rutin Obtained from Polygala paniculata L. in MiceBasic & Clinical Pharmacology & Toxicology, 2009
- Phenolic compounds and antioxidant activities of someHypericumspecies: A comparative study withH. perforatumPharmaceutical Biology, 2009
- Effects of acute or 3-day treatments of Hypericum caprifoliatum Cham. & Schltdt. (Guttiferae) extract or of two established antidepressants on basal and stress-induced increase in serum and brain corticosterone levelsJournal of Psychopharmacology, 2008
- Anticonvulsive effects of rutin in a rat model of absence seizure: a novel compound to treat seizureAnnals of General Psychiatry, 2008
- Differential effects of three species of Hypericum in an open field testPhytotherapy Research, 2007
- Caffeic acid produces antidepressive- and/or anxiolytic-like effects through indirect modulation of the α1A-adrenoceptor system in miceNeuroReport, 2003