Induction of vacuolar CaZ+-ATPase and H+/Ca 2+exchange activity in yeast mutants lacking Pmrl, the Golgi Ca2+-ATPase
Open Access
- 9 July 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 454 (3), 181-186
- https://doi.org/10.1016/s0014-5793(99)00803-0
Abstract
We have analyzed Ca2+ transport activity in defined subcellular fractions of an isogenic set of wild-type and mutant yeast. The results, together with measurements of polypeptide expression levels and promoter::reporter gene activity, show that the Golgi Ca2+-ATPase, Pmr1, is the major Ca2+ pump under normal growth conditions. In the absence of Pmr1, we show a massive, calcineurin-dependent compensatory induction of the vacuolar Ca2+-ATPase, Pmc1. In addition, H+/Ca2+ exchange activity, that may be distinct from the vacuolar exchanger Vcxl, is also increasedKeywords
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