Combination of Hypoosmotic Swelling/Eosin Y Test for Sperm Membrane Integrity Evaluation: Correlations with Other Sperm Parameters to Predict ICSI Cycles
- 1 January 2007
- journal article
- Published by Taylor & Francis Ltd in Archives of Andrology
- Vol. 53 (1), 25-28
- https://doi.org/10.1080/01485010600888912
Abstract
The objective of our study was to evaluate the accuracy of the combination of hypoosmotic swelling (HOS) and eosin Y (Ey) exclusion tests to predict the ICSI cycles' outcome and its correlations with other sperm parameters. The functional and structural integrity of sperm membrane was evaluated with the combined HOS/Ey test in 95 ICSI cycles and the results were correlated with other sperm parameters, including concentration, motility, strict morphology, and total motile sperm count. The combined HOS/Ey test was evaluated for the prediction of the ICSI cycles' outcome parameters including fertilization, cleavage, and pregnancy rates. The HOS/Ey test presented significant relationships with concentration, motility, and strict morphology (p < 0,0001) but it couldn't predict the fertilization, cleavage, and pregnancy outcomes of ICSI cycles. The combined HOS/Ey test has strong correlations with motility and strict morphology parameters of sperm samples but is not sufficiently sensitive to estimate the outcome of ICSI cycles.Keywords
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