A shear-inducedin vitroplatelet function test can assess clinically relevant anti-thrombotic effects
- 1 January 1999
- journal article
- research article
- Published by Informa UK Limited in Platelets
- Vol. 10 (2-3), 178-184
- https://doi.org/10.1080/09537109909169182
Abstract
Morphological features of haemostatic plugs formed in vitro under high shear forces were investigated. Electron microscopy confirmed the relevance of such haemostatic plug to a platelet-rich arterial thrombus, which is formed in vivo. In rat blood samples, the effects of anticoagulants and various antiplatelet agents on platelet reactivity (rate of haemostatic plug formation) and subsequent coagulation of the flowing blood were investigated. Haemostasis did not occur in citrated blood, and heparin greatly inhibited the shear-induced platelet reaction. Aspirin (1 mM), a thromboxane A2 receptor antagonist (5 μM), a stable prostacyclin (0.55 nM), a stable prostaglandin E1 (141 nM) and a phosphodiesterase inhibitor (100 μM) were tested. All these agents exerted significant inhibitory effect on shear-induced platelet reaction, including the inhibition of the very first phase of platelet plug formation, due to aggregation of shear-activated platelets. Except for the phosphodiesterase inhibitor, which prolonged clotting time, none of the above agents affected dynamic coagulation. These results suggest that the employed in vitro shear-induced thrombosis/haemostasis test can reveal in vivo the antithrombotic effect of various agents independently of their mechanism of action.Keywords
This publication has 22 references indexed in Scilit:
- Thrombin hypothesis of thrombus generation and vascular lesion formationThe American Journal of Cardiology, 1995
- A perfusion chamber developed to investigate thrombus formation and shear profiles in flowing native human blood at the apex of well-defined stenoses.Arteriosclerosis and Thrombosis: A Journal of Vascular Biology, 1994
- Shear-induced platelet aggregation in cerebral ischemia.Stroke, 1994
- The antithrombotic effect of low molecular weight synthetic thrombin inhibitors, argatroban and PPACK, on He-Ne laser-induced thrombosis in rat mesenteric microvesselsThrombosis Research, 1993
- High-dose aspirin inhibits shear-induced platelet reaction involving thrombin generation.Circulation, 1992
- The role of von Willebrand factor and fibrinogen in platelet aggregation under varying shear stress.JCI Insight, 1991
- Enhancement of platelet reactivity and modulation of eicosanoid production by intact erythrocytes. A new approach to platelet activation and recruitment.JCI Insight, 1991
- Altered platelet function during mental stress and adrenaline infusion in humans: evidence for an increased aggregability in vivo as measured by filtragometryClinical Science, 1989
- Haemostatometer: A new in vitro technique for assessing haemostatic activity of bloodThrombosis Research, 1984
- Analysis of pulsatile, viscous blood flow through diseased coronary arteries of manJournal of Biomechanics, 1977