Coinfection withHaemophilus influenzaePromotes Pneumococcal Biofilm Formation during Experimental Otitis Media and Impedes the Progression of Pneumococcal Disease
Open Access
- 1 October 2010
- journal article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 202 (7), 1068-1075
- https://doi.org/10.1086/656046
Abstract
Background. Otitis media is an extremely common pediatric infection and is mostly caused by bacteria that are carried within the nasopharyngeal microbiota. It is clear that most otitis media cases involve simultaneous infection with multiple agents. Methods. Chinchillas were infected with nontypeable Haemophilus influenzae, Streptococcus pneumoniae, or a combination of both organisms, and the course of disease was compared. In vitro experiments were also performed to address how coinfection impacts biofilm formation. Results. The incidence of systemic disease was reduced in coinfected animals, compared with those infected with pneumococcus alone. Pneumococci were present within surface-attached biofilms in coinfected animals, and a greater proportion of translucent colony type was observed in the coinfected animals. Because this colony type has been associated with pneumococcal biofilms, the impact of coinfection on pneumococcal biofilm formation was investigated. The results clearly show enhanced biofilm formation in vitro by pneumococci in the presence of H. influenzae. Conclusions. Based on these data, we conclude that coinfection with H. influenzae facilitates pneumococcal biofilm formation and persistence on the middle ear mucosal surface. This enhanced biofilm persistence correlates with delayed emergence of opaque colony variants within the bacterial population and a resulting decrease in systemic infection.Keywords
This publication has 29 references indexed in Scilit:
- Sialic Acid: A Preventable Signal for Pneumococcal Biofilm Formation, Colonization, and Invasion of the HostThe Journal of Infectious Diseases, 2009
- Streptococcus pneumoniaeForms Surface‐Attached Communities in the Middle Ear of Experimentally Infected ChinchillasThe Journal of Infectious Diseases, 2009
- Survival of Bacterial Biofilms within Neutrophil Extracellular Traps Promotes Nontypeable Haemophilus influenzae Persistence in the Chinchilla Model for Otitis MediaJournal of Innate Immunity, 2009
- Phosphorylcholine Expression by NontypeableHaemophilus influenzaeCorrelates with Maturation of Biofilm Communities In Vitro and In VivoJournal of Bacteriology, 2007
- Characterization of Colony Morphology Variants Isolated fromStreptococcus pneumoniaeBiofilmsJournal of Bacteriology, 2007
- Phosphorylcholine Decreases Early Inflammation and Promotes the Establishment of Stable Biofilm Communities of Nontypeable Haemophilus influenzae Strain 86-028NP in a Chinchilla Model of Otitis MediaInfection and Immunity, 2007
- Role of Sialic Acid and Complex Carbohydrate Biosynthesis in Biofilm Formation by Nontypeable Haemophilus influenzae in the Chinchilla Middle EarInfection and Immunity, 2005
- Nontypeable Haemophilus influenzae Strain 2019 Produces a Biofilm Containing N -Acetylneuraminic Acid That May Mimic Sialylated O-Linked GlycansInfection and Immunity, 2004
- Complete Genome Sequence of a Virulent Isolate of Streptococcus pneumoniaeScience, 2001
- Purine metabolism in Neisseria gonorrhoeae: the requirement for hypoxanthineCanadian Journal of Microbiology, 1980