Human Papillomavirus Infection Requires Cell Surface Heparan Sulfate
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- 1 February 2001
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 75 (3), 1565-70
- https://doi.org/10.1128/jvi.75.3.1565-1570.2001
Abstract
Using pseudoinfection of cell lines, we demonstrate that cell surface heparan sulfate is required for infection by human papillomavirus type 16 (HPV-16) and HPV-33 pseudovirions. Pseudoinfection was inhibited by heparin but not dermatan or chondroitin sulfate, reduced by reducing the level of surface sulfation, and abolished by heparinase treatment. Carboxy-terminally deleted HPV-33 virus-like particles still bound efficiently to heparin. The kinetics of postattachment neutralization by antiserum or heparin indicated that pseudovirions were shifted on the cell surface from a heparin-sensitive into a heparin-resistant mode of binding, possibly involving a secondary receptor. Alpha-6 integrin is not a receptor for HPV-33 pseudoinfection.Keywords
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