Combi-protein coated microcrystals of lipases for production of biodiesel from oil from spent coffee grounds
Open Access
- 8 August 2013
- journal article
- Published by Springer Science and Business Media LLC in Sustainable Chemical Processes
- Vol. 1 (1), 14
- https://doi.org/10.1186/2043-7129-1-14
Abstract
Replacing chemical catalysts with biocatalysts is a widely recognized goal of white biotechnology. For biocatalytic processes requiring low water containing media, enzymes for example commercial preparations of lipases, show low catalytic efficiencies. Some high activity preparations for addressing this concern have been described. Protein coated microcrystals (PCMC) constitute one such preparation. The present work describes a Combi-PCMC for synthesis of biodiesel from the oil extracted from spent coffee grounds. Different lipases were screened for biodiesel synthesis from crude coffee oil out of which Novozym 435 gave the best conversion of 60% in 4 h. Optimization of reaction conditions i.e. % water, temperature and purification of coffee oil further enhanced conversion upto 88% in 24 h. A mixture of Novozym 435 and a cheap commercially available 1,3-specific lipase RMIM (from Mucor miehei) was used in different ratios and 1:1 was found to be the best trade-off between conversion and cost. The commercial preparations then were replaced by a novel biocatalyst design called Combi-Protein coated microcrystals (Combi-PCMC) wherein CAL B and Palatase were co-immobilized with K2SO4 as the core and this performed equivalent to the commercial preparations giving 83% conversion in 48 h. Coffee oil extracted from spent coffee grounds could be used for the synthesis of biodiesel by using appropriate commercial preparations of lipases. The expensive commercially immobilized preparations can also be replaced by a simpler and inexpensive immobilization design called combi-PCMC which synergizes the catalytic action of a nonspecific lipase CAL B and a free form of 1,3-specific lipase from Mucor miehei.Keywords
This publication has 69 references indexed in Scilit:
- Supercritical Synthesis of BiodieselMolecules, 2012
- Increasing the catalytic efficiency ofCandida rugosalipase for the synthesis oftert-alkyl butyrates in low-water mediaBiocatalysis and Biotransformation, 2011
- Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs)Applied Microbiology and Biotechnology, 2011
- Biodiesel production with immobilized lipase: A reviewBiotechnology Advances, 2010
- Biocatalysis: Towards ever greener biodiesel productionBiotechnology Advances, 2009
- Biodiesel production from rapeseed deodorizer distillate in a packed column reactorChemical Engineering and Processing - Process Intensification, 2009
- Biodiesel from waste cooking oil via base-catalytic and supercritical methanol transesterificationEnergy Conversion and Management, 2009
- A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activitiesChemistry Central Journal, 2007
- Lipase catalyzed preparation of biodiesel from Jatropha oil in a solvent free systemProcess Biochemistry, 2007
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976