Detection and Genotype of High-Risk Human Papillomavirus in Fine-Needle Aspirates of Patients With Metastatic Squamous Cell Carcinoma Is Helpful in Determining Tumor Origin
Open Access
- 1 May 2015
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 143 (5), 694-700
- https://doi.org/10.1309/ajcpcza4pszcfhq4
Abstract
Recent studies have shown that human papillomavirus (HPV) is associated with a certain subset of head and neck squamous cell carcinoma (HNSCC)—namely, those arising in the oropharynx. The objective of this study is to determine the efficacy, detection, and genotype of high-risk (HR) HPV using the Roche cobas 4800 system (Roche Molecular System, Pleasanton, CA). Forty-two fine-needle aspirate (FNA) specimens from 37 patients with cervical (n = 36) or mediastinal (n = 5) lymphadenopathy or a left parapharyngeal mass (n =1) were included in this prospective study. HR-HPV testing was performed on residual FNA material after direct smear preparation and, if positive, was further delineated into HPV 16/18 genotypes using the Roche cobas 4800 system. Follow-up included review of histologic material and/or electronic health records. Among those HNSCCs that were positive for HR-HPV, 18 (100%) of 18 originated from the oropharynx, whereas only two (13%) of 15 HR-HPV–negative HNSCCs originated from the oropharynx (χ2 test, P < .05). p16 immunohistochemical assay and HPV 16 in situ hybridization on corresponding histologic specimens were concordant with cytologic HR-HPV results. HR-HPV detection and genotyping can be performed on lymph node FNAs with metastatic squamous cell carcinoma using the Roche cobas 4800 system. The presence of HR-HPV and/or HPV 16 is a reliable indicator of the metastatic squamous cell carcinoma originating from the oropharynx.Keywords
This publication has 31 references indexed in Scilit:
- Adequacy of fine-needle aspiration specimens for human papillomavirus infection molecular testing in head and neck squamous cell carcinomaCytojournal, 2013
- Human Papillomavirus-Related Small Cell Carcinoma of the OropharynxThe American Journal of Surgical Pathology, 2011
- Application of the Hybrid Capture 2 assay to squamous cell carcinomas of the head and neckCancer Cytopathology, 2011
- Lymphoepithelial-like Carcinoma of the OropharynxThe American Journal of Surgical Pathology, 2010
- Assessing for primary oropharyngeal or nasopharyngeal squamous cell carcinoma from fine needle aspiration of cervical lymph node metastasesDiagnostic Cytopathology, 2009
- Papillary Squamous Cell Carcinoma of the Head and NeckThe American Journal of Surgical Pathology, 2009
- Incidence Trends for Human Papillomavirus–Related and –Unrelated Oral Squamous Cell Carcinomas in the United StatesJournal of Clinical Oncology, 2008
- Detection of Human Papillomavirus-16 in Fine-Needle Aspirates to Determine Tumor Origin in Patients with Metastatic Squamous Cell Carcinoma of the Head and NeckClinical Cancer Research, 2007
- Diagnostic work-up and outcome of cervical metastases from an unknown primaryActa Oto-Laryngologica, 2006
- Human papillomavirus genome detection by in situ hybridization in fine-needle aspirates of metastatic lesions from head and neck squamous cell carcinomasCancer, 2005