Evaluation and differentiation of the Betulaceae birch bark species and their bioactive triterpene content using analytical FT-vibrational spectroscopy and GC-MS
Open Access
- 18 July 2012
- journal article
- Published by Springer Science and Business Media LLC in Chemistry Central Journal
- Vol. 6 (1), 67
- https://doi.org/10.1186/1752-153x-6-67
Abstract
Aiming to obtain the highest triterpene content in the extraction products, nine bark samples from the forest abundant flora of Apuseni Mountains, Romania were Raman spectroscopically evaluated. Three different natural extracts from Betula pendula Roth birch bark have been obtained and characterized using Fourier transform vibrational spectra. This study shows that principal components of the birch tree extract can be rapidly recognized and differentiated based on their vibrational fingerprint band shape and intensity. The vibrational spectroscopy results are supported by the GC-MS data. Based on IR and Raman analysis, one can conclude that all the extracts, independent on the solvent(s) used, revealed dominant betulin species, followed by lupeol. Since Raman measurements could also be performed on fresh plant material, we demonstrated the possibility to apply the present results for the prediction of the highest triterpene content in bark species, for the selection of harvesting time or individual genotypes directly in the field, with appropriate portable Raman equipment.Keywords
This publication has 20 references indexed in Scilit:
- Betulin and its natural resource as potential anticancer drug candidate seen by FT‐Raman and FT‐IR spectroscopyJournal of Raman Spectroscopy, 2011
- Recent advances in linear and nonlinear Raman spectroscopy. Part IVJournal of Raman Spectroscopy, 2010
- Recent advances in linear and non‐linear Raman spectroscopy. Part IIIJournal of Raman Spectroscopy, 2009
- Recent advances in linear and nonlinear Raman spectroscopy IIJournal of Raman Spectroscopy, 2008
- Recent Advances in linear and nonlinear Raman spectroscopy IJournal of Raman Spectroscopy, 2007
- Simultaneous determination of betulin and betulinic acid in white birch bark using RP-HPLCJournal of Pharmaceutical and Biomedical Analysis, 2007
- Identification and quantification of valuable plant substances by IR and Raman spectroscopyVibrational Spectroscopy, 2007
- In vitro studies on mesenchymal stem cells of a dry birch tree bark extractToxicology Letters, 2006
- Birch bark research and developmentNatural Product Reports, 2006
- Chemistry, biological activity, and chemotherapeutic potential of betulinic acid for the prevention and treatment of cancer and HIV infectionMedicinal Research Reviews, 2003