Purification and sequencing of cerein 7B, a novel bacteriocin produced byBacillus cereusBc7
Open Access
- 1 January 2006
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 254 (1), 108-115
- https://doi.org/10.1111/j.1574-6968.2005.00009.x
Abstract
Cerein 7B is a new bacteriocin produced simultaneously with cerein 7A by Bacillus cereus Bc7 in liquid brain heart infusion cultures. Both bacteriocins are not synergistic. The two peptides have been purified to homogeneity by hydrophobic interaction, cation exchange and reverse‐phase liquid chromatography. They can be distinguished by their N‐terminal amino acid sequences N‐Gly‐Trp‐Gly‐Asp‐Val‐Leu (7A) and N‐Gly‐Trp‐Trp‐Asn‐Ser‐Trp‐Gly‐Lys (7B). Pre‐cerein 7B is 74 amino acids long and contains an 18 aminoacid double‐glycine type leader sequence that is removed to produce the mature bacteriocin. The leader peptide sequence is related to that of sec‐independent secretion signals suggesting that cerein 7B belongs to class II sec‐independent bacteriocins.Keywords
This publication has 37 references indexed in Scilit:
- Purification and partial chemical characterization of the antimicrobial peptide cerein 8ALetters in Applied Microbiology, 2005
- Characterization of Bacillus Probiotics Available for Human UseApplied and Environmental Microbiology, 2004
- Differential Roles of the Two-Component Peptides of Lactocin 705 in Antimicrobial ActivityCurrent Microbiology, 2003
- Mutational Analysis of the Role of Tryptophan Residues in an Antimicrobial PeptideBiochemistry, 2002
- Characterization of the genetic locus responsible for production and immunity of carnobacteriocin A: the immunity gene confers cross-protection to enterocin B The GenBank/EMBL/DDBJ accession number for the sequence reported in this paper is AF207838Microbiology, 2000
- Plantaricin D, a bacteriocin produced by Lactobacillus plantarum BFE 905 from ready-to-eat saladLetters in Applied Microbiology, 1998
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin AMicrobiology, 1997
- Characterization of the locus responsible for the bacteriocin production in Lactobacillus plantarum C11Journal of Bacteriology, 1996
- Evidence that the amyloid fibril protein in senile systemic amyloidosis is derived from normal prealbuminBiochemical and Biophysical Research Communications, 1988