Antioxidant activity of hyaluronic acid investigated by means of chemiluminescence of equine neutrophil bursts and electron paramagnetic resonance spectroscopy
- 28 July 2014
- journal article
- Published by Wiley in Journal of Veterinary Pharmacology and Therapeutics
- Vol. 38 (1), 48-54
- https://doi.org/10.1111/jvp.12141
Abstract
Activated neutrophils (PMNs), the ROS/RNS released by PMNs and the derived inflammatory processes are involved in the pathogenesis and progression of human inflammatory airway diseases. Similar diseases are also present in horses which suffer from recurrent airway obstruction (RAO), exercise-induced pulmonary haemorrhage (EIPH) and inflammatory airway diseases (IAD). Hyaluronic acid (HA) plays numerous roles in modulating inflammatory processes. The aim of this study was to examine whether a preparation of HA (MW 900 000 Da) interferes with ROS/RNS during the course of equine PMN respiratory bursts, and to establish the lowest concentration at which it still has antioxidant activity by means of luminol-amplified chemiluminescence (LACL). Electron paramagnetic resonance (EPR) spectroscopy was also used to investigate the direct antiradical activity of HA. The hydroxyl radical was significantly scavenged in a concentration-dependent manner at HA concentrations ranging from 2.5 to 0.16 mg/mL. Superoxide anion, Tempol radical and the ABTS(•+) were significantly inhibited at concentrations ranging from 2.5 to 0.62 mg/mL. The LACL of stimulated equine neutrophils showed that HA induced a statistically significant concentration-effect reduction from 5 mg/mL to 1.25 mg/mL. These findings were confirmed also when l-Arg was added to investigate the inhibition of the resulting peroxynitrite anion. Our findings indicate that, in addition to the human use, HA can also be used to antagonize the oxidative stress generated by free radicals in horses peripheral blood mononuclear cells (PBMCs). In order to achieve therapeutic concentrations, a direct aerosol administration to horses with horse respiratory diseases can be considered, as this route of application is also recommended in human medicine.Keywords
This publication has 29 references indexed in Scilit:
- Antioxidant Activity of Calendula officinalis Extract: Inhibitory Effects on Chemiluminescence of Human Neutrophil Bursts and Electron Paramagnetic Resonance SpectroscopyPharmacology, 2009
- Evaluation of polysulfated glycosaminoglycan or sodium hyaluronan administered intra-articularly for treatment of horses with experimentally induced osteoarthritisAmerican Journal of Veterinary Research, 2009
- CD44-specific antibody treatment and CD44 deficiency exert distinct effects on leukocyte recruitment in experimental arthritisBlood, 2008
- Comparison of Cardioprotective Abilities between the Flesh and Skin of GrapesJournal of Agricultural and Food Chemistry, 2006
- Comparison of the Radical Scavenging Potential of Polar and Lipidic Fractions of Olive Oil and Other Vegetable Oils under Normal Conditions and after Thermal TreatmentJournal of Agricultural and Food Chemistry, 2004
- Effects of Hyaluronic Acid on the Polymorphonuclear Leukocyte (PMN) Release of Active Oxygen and Protection of Bovine Corneal Endothelial Cells from Activated PMNsKorean Journal of Ophthalmology, 2004
- Plasma and bronchoalveolar fluid concentrations of nitric oxide and localization of nitric oxide synthesis in the lungs of horses with summer pastureassociated obstructive pulmonary diseaseAmerican Journal of Veterinary Research, 2001
- Effects of hyaluronic acid on macrophage phagocytosis and active oxygen releaseInflammation Research, 1993
- Spin-Trapping of the Superoxide Radical in Aprotic SolventsFree Radical Research Communications, 1991
- Regulation of granulocyte function by hyaluronic acid. In vitro and in vivo effects on phagocytosis, locomotion, and metabolism.JCI Insight, 1980