Genotypic characteristics of Mycobacterium tuberculosis isolated from household contacts of tuberculosis patients in the Philippines
Open Access
- 5 December 2013
- journal article
- Published by Springer Science and Business Media LLC in BMC Infectious Diseases
- Vol. 13 (1), 571
- https://doi.org/10.1186/1471-2334-13-571
Abstract
The Philippines has an extremely high rate of tuberculosis but little is known about M. tuberculosis genotypes and transmission dynamics in this country. The aim of this study was to determine the proportion of household contacts who develop active TB due to direct transmission from an index case in that household. Mycobacterium tuberculosis isolates from household contacts of tuberculosis patients in the Philippines were characterized using restriction-fragment-length polymorphism analysis, spoligotyping, and mycobacterial interspersed repetitive units – variable number tandem repeats typing (12-loci) to determine their utility in elucidating transmission in an area of high tuberculosis prevalence. Drug susceptibility patterns for these isolates were also determined. Spoligotyping and MIRU-VNTR typing results matched in 10 (62.5%) of 16 index patient-household contact pairs while IS6110 fingerprints matched in only six (37.5%) pairs. Only 3/16 (18.8%) index patient-household contact pairs had identical drug susceptibility results. Strain typing of M. tuberculosis isolates from household contacts in the Philippines indicates that transmission of strains does not necessarily occur directly from the index patient living in close proximity in the same household but rather that community-based transmission also frequently occurs. Accurate susceptibility testing of all isolates is necessary to insure optimal care of both the index patients and any culture-positive household contacts.Keywords
This publication has 22 references indexed in Scilit:
- First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex StrainsJournal of Clinical Microbiology, 2012
- Incidence of Tuberculosis and the Predictive Value of ELISPOT and Mantoux Tests in Gambian Case ContactsPLOS ONE, 2008
- Assessment of an Optimized Mycobacterial Interspersed Repetitive- Unit-Variable-Number Tandem-Repeat Typing System Combined with Spoligotyping for Population-Based Molecular Epidemiology Studies of TuberculosisJournal of Clinical Microbiology, 2007
- Proposal for Standardization of Optimized Mycobacterial Interspersed Repetitive Unit-Variable-Number Tandem Repeat Typing ofMycobacterium tuberculosisJournal of Clinical Microbiology, 2006
- The burden of disease, economic costs and clinical consequences of tuberculosis in the PhilippinesHealth Policy and Planning, 2005
- Molecular Epidemiology: A Tool for Understanding Control of Tuberculosis TransmissionClinics in Chest Medicine, 2005
- Evaluation of a Two-Step Approach for Large-Scale, Prospective Genotyping ofMycobacterium tuberculosisIsolates in the United StatesJournal of Clinical Microbiology, 2005
- Utility of Fast Mycobacterial Interspersed Repetitive Unit--Variable Number Tandem Repeat Genotyping in Clinical Mycobacteriological AnalysisClinical Infectious Diseases, 2004
- Characterization of the Manila Family of Mycobacterium tuberculosisJournal of Clinical Microbiology, 2003
- Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeatsMicrobiology, 1998