Kinetics and Thermodynamics of Dissolution of Lecithin by Bile Salts

Abstract

The kinetics of dissolution of dispersions of egg phosphatidylcholine (lecithin) by bile salts was studied by observing the decrease in turbidity as mixed micelles of lecithin and bile salts were formed. The rate of dissolution of lecithin corresponding to formation of mixed micelles was studied in the presence of dihydroxy bile salts, sodium deoxycholate, sodium chenodeoxycholate, sodium ursodeoxycholate and one trihydroxy bile salt, sodium cholate. The rate of dissolution of lecithin and mixed micelle formation decreased in the order: chenodeoxycholate ± deoxycholate ± cholate ± ursodeoxycholate. Kinetic solvent isotope studies in D₂O, along with measurement of enthalpies of mixed micelle formation both in H₂O and D₂O, suggest that formation and stabilization of mixed micelles are related to “hydrophobicity” as estimated by high performance liquid chromatography retention factors.