Functional heterogeneity of human memory CD4 + T cell clones primed by pathogens or vaccines
- 23 January 2015
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 347 (6220), 400-406
- https://doi.org/10.1126/science.1260668
Abstract
Distinct types of CD4+ T cells protect the host against different classes of pathogens. However, it is unclear whether a given pathogen induces a single type of polarized T cell. By combining antigenic stimulation and T cell receptor deep sequencing, we found that human pathogen- and vaccine-specific T helper 1 (TH1), TH2, and TH17 memory cells have different frequencies but comparable diversity and comprise not only clones polarized toward a single fate, but also clones whose progeny have acquired multiple fates. Single naïve T cells primed by a pathogen in vitro could also give rise to multiple fates. Our results unravel an unexpected degree of interclonal and intraclonal functional heterogeneity of the human T cell response and suggest that polarized responses result from preferential expansion rather than priming.Keywords
Funding Information
- European Research Council (323183 PREDICT)
- European Commission (FP7-HEALTH-2011-280873, FP7-HEALTH-2013-601958)
- Swiss National Science Foundation (149475, 147662)
- Helmut Horten Foundation.
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