Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy.
Open Access
- 11 October 1994
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 91 (21), 10109-10113
- https://doi.org/10.1073/pnas.91.21.10109
Abstract
Transgenic mice were generated by using the alpha-myosin heavy chain promoter coupled to the coding sequence of a constitutively active mutant alpha 1B-adrenergic receptor (AR). These transgenic animals demonstrated cardiac-specific expression of this alpha 1-AR with resultant activation of phospholipase C as shown by increased myocardial diacylglycerol content. A phenotype consistent with cardiac hypertrophy developed in adult transgenic mice with increased heart/body weight ratios, myocyte cross-sectional areas, and ventricular atrial natriuretic factor mRNA levels relative to nontransgenic controls. These transgenic animals may provide insight into the biochemical triggers that induce hypertrophy in cardiac disease and serve as a convenient experimental model for studies of this condition.Keywords
This publication has 27 references indexed in Scilit:
- Transcriptional Regulation During Cardiac Growth and DevelopmentAnnual Review of Physiology, 1993
- Targeted developmental overexpression of calmodulin induces proliferative and hypertrophic growth of cardiomyocytes in transgenic mice.Endocrinology, 1993
- Alpha 1-adrenoceptors in myocardium: functional aspects and transmembrane signaling mechanisms.Physiological Reviews, 1993
- Endothelin induction of inositol phospholipid hydrolysis, sarcomere assembly, and cardiac gene expression in ventricular myocytes. A paracrine mechanism for myocardial cell hypertrophyJournal of Biological Chemistry, 1990
- Regions of the alpha 1-adrenergic receptor involved in coupling to phosphatidylinositol hydrolysis and enhanced sensitivity of biological function.Proceedings of the National Academy of Sciences of the United States of America, 1990
- Protooncogene induction and reprogramming of cardiac gene expression produced by pressure overload.Proceedings of the National Academy of Sciences, 1988
- A hemodynamic load in vivo induces cardiac expression of the cellular oncogene, c-mycBiochemical and Biophysical Research Communications, 1987
- Cardiac myocyte hypertrophy is associated with c-myc protooncogene expression.Proceedings of the National Academy of Sciences of the United States of America, 1986
- Quantitative measurement of sn-1,2-diacylglycerols present in platelets, hepatocytes, and ras- and sis-transformed normal rat kidney cells.Journal of Biological Chemistry, 1986
- Norepinephrine-stimulated hypertrophy of cultured rat myocardial cells is an alpha 1 adrenergic response.JCI Insight, 1983