Development of an Enzyme-Linked Immunosorbent Assay for the Pyrethroid Insecticide Cyhalothrin
- 23 June 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 54 (15), 5284-5291
- https://doi.org/10.1021/jf0607009
Abstract
A competitive enzyme-linked immunosorbent assay (ELISA) was developed for detection of the pyrethroid insecticide cyhalothrin. Three haptens with an amine or propanoic acid terminus were synthesized and then conjugated with bovine serum albumin to give immunogens. Eight polyclonal antisera produced by rabbits were screened for titers and affinity using three different coating antigens. The antiserum CWB-C had the highest affinity with cyhalothrin and a low affinity with fenvalerate, fenpropathrin, deltamethrin, and fluvalinate. The half-maximum inhibition concentration for cyhalothrin was 37.2 μg/L, and the limit of detection was 4.7 μg/L. The recoveries of different concentrations of cyhalothrin (0.1−2500 μg/L) from fortified tap water, well water, and wastewater samples as determined with the ELISA were 81−114%. Keywords: ELISA; pyrethroid; cyhalothrinKeywords
This publication has 12 references indexed in Scilit:
- Development of a class-specific immunoassay for the type I pyrethroid insecticidesAnalytica Chimica Acta, 2001
- A monoclonal antibody‐based ELISA for the analysis of the insecticide flucythrinate in environmental and crop samplesPest Management Science, 2001
- Monitoring of pyrethroid metabolites in human urine using solid-phase extraction followed by gas chromatography-tandem mass spectrometryAnalytica Chimica Acta, 1999
- Development of an Enzyme-Linked Immunosorbent Assay for the Detection of the Pyrethroid Insecticide FenpropathrinJournal of Agricultural and Food Chemistry, 1998
- Homogeneous enzyme immunoassay for pyrethroid pesticides and their derivatives using bacillary alpha-amylase as labelAnalytica Chimica Acta, 1997
- Insecticides as Tools in Probing Vital Receptors and Enzymes in Excitable MembranesPesticide Biochemistry and Physiology, 1997
- Determination of Non-extractable Triazine Residues by Enzyme Immunoassay: Investigation of Model Compounds and Soil Fulvic and Humic AcidsEnvironmental Science & Technology, 1996
- Reaction of 20S Proteasome: Shift of SDS-Dependent Activation Profile by Divalent Cations1The Journal of Biochemistry, 1995
- Analysis of the synthetic pyrethroids, permethrin and 1(R)-phenothrin, in grain using a monoclonal antibody-based testJournal of Agricultural and Food Chemistry, 1992
- Extraction efficiencies for pesticides in crops. 1. [14C]Carbaryl extraction from mustard greens and radishesJournal of Agricultural and Food Chemistry, 1978