Oligodendroglial cells in culture effectively dispose of exogenous hydrogen peroxide: comparison with cultured neurones, astroglial and microglial cells
- 24 July 2002
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 82 (3), 635-644
- https://doi.org/10.1046/j.1471-4159.2002.00999.x
Abstract
To investigate the antioxidative capacities of oligodendrocytes, rat brain cultures enriched for oligodendroglial cells were prepared and characterized. These cultures contained predominantly oligodendroglial cells as determined by immunocytochemical staining for the markers galactocerebroside and myelin basic protein. If oligodendroglial cultures were exposed to exogenous hydrogen peroxide (100 µm), the peroxide disappeared from the incubation medium following first order kinetics with a half‐time of approximately 18 min. Normalization of the disposal rate to the protein content of the cultures by calculation of the specific hydrogen peroxide detoxification rate constant revealed that the cells in oligodendroglial cultures have a 60% to 120% higher specific capacity to dispose of hydrogen peroxide than cultures enriched for astroglial cells, microglial cells or neurones. Oligodendroglial cultures contained specific activities of 133.5 ± 30.4 nmol × min−1 × mg protein−1 and 27.5 ± 5.4 nmol × min−1 × mg protein−1 of glutathione peroxidase and glutathione reductase, respectively. The specific rate constant of catalase in these cultures was 1.61 ± 0.54 min−1 × mg protein−1. Comparison with data obtained by identical methods for cultures of astroglial cells, microglial cells and neurones revealed that all three of the enzymes which are involved in hydrogen peroxide disposal were present in oligodendroglial cultures in the highest specific activities. These results demonstrate that oligodendroglial cells in culture have a prominent machinery for the disposal of hydrogen peroxide, which is likely to support the protection of these cells in brain against peroxides when produced by these or by surrounding brain cells.Keywords
This publication has 48 references indexed in Scilit:
- Delayed oligodendrocyte degeneration induced by brief exposure to hydrogen peroxideJournal of Neuroscience Research, 1999
- Mode of Cell Injury and Death after Hydrogen Peroxide Exposure in Cultured Oligodendroglia CellsExperimental Cell Research, 1998
- Antioxidant defense of the brain: a role for astrocytesCanadian Journal of Physiology and Pharmacology, 1997
- Response of Glial Cells to Ischemia: Roles of Reactive Oxygen Species and GlutathioneNeuroscience & Biobehavioral Reviews, 1997
- Glutathione Content as an Indicator for the Presence of Metabolic Pathways of Amino Acids in Astroglial CulturesJournal of Neurochemistry, 1996
- Cystine Deprivation Induces Oligodendroglial Death: Rescue by Free Radical Scavengers and by a Diffusible Glial FactorJournal of Neurochemistry, 1996
- Immunocytochemical localization of catalase in the central nervous system of the rat.Journal of Histochemistry & Cytochemistry, 1995
- Ethanol-Induced Increase in Catalase Activity in Reaggregation Cultures of Rat Brain Cells is Due to Increased Oligodendrocyte DifferentiationAlcohol: Clinical and Experimental Research, 1994
- Purification of Cytosolic Malic Enzyme from Bovine Brain, Generation of Monoclonal Antibodies, and Immunocytochemical Localization of the Enzyme in Glial Cells of Neural Primary CulturesJournal of Neurochemistry, 1993
- Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue.The Journal of cell biology, 1980