Enhanced plant tolerance to iron starvation by functional substitution of chloroplast ferredoxin with a bacterial flavodoxin
- 3 July 2007
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences of the United States of America
- Vol. 104 (27), 11495-11500
- https://doi.org/10.1073/pnas.0704553104
Abstract
Iron limitation affects one-third of the cultivable land on Earth and represents a major concern for agriculture. It causes decline of many photosynthetic components, including the Fe-S protein ferredoxin (Fd), involved in essential oxidoreductive pathways of chloroplasts. In cyanobacteria and some algae, Fd down-regulation under Fe deficit is compensated by induction of an isofunctional electron carrier, flavodoxin (Fld), a flavin mononucleotide-containing protein not found in plants. Transgenic tobacco lines expressing a cyanobacterial Fld in chloroplasts were able to grow in Fe-deficient media that severely compromised survival of WT plants. Fld expression did not improve Fe uptake or mobilization, and stressed transformants elicited a normal deficit response, including induction of ferric-chelate reductase and metal transporters. However, the presence of Fld did prevent decrease of several photosynthetic proteins (but not Fd) and partially protected photosynthesis from inactivation. It also preserved the activation state of enzymes depending on the Fd-thioredoxin pathway, which correlated with higher levels of intermediates of carbohydrate metabolism and the Calvin cycle, as well as increased contents of sucrose, glutamate, and other amino acids. These metabolic routes depend, directly or indirectly, on the provision of reduced Fd. The results indicate that Fld could compensate Fd decline during episodes of Fe deficiency by productively interacting with Fd-dependent pathways of the host, providing fresh genetic resources for the design of plants able to survive in Fe-poor lands.This publication has 40 references indexed in Scilit:
- Functional Replacement of Ferredoxin by a Cyanobacterial Flavodoxin in Tobacco Confers Broad-Range Stress ToleranceTHE PLANT CELL ONLINE, 2006
- Decreased Content of Leaf Ferredoxin Changes Electron Distribution and Limits Photosynthesis in Transgenic Potato PlantsPlant Physiology, 2003
- Combined deficiency of iron and other divalent cations mitigates the symptoms of iron deficiency in tobacco plantsPhysiologia Plantarum, 2003
- Differential Regulation of nramp and irt Metal Transporter Genes in Wild Type and Iron Uptake Mutants of TomatoOnline Journal of Public Health Informatics, 2003
- Response of Arabidopsis to Iron Deficiency Stress as Revealed by Microarray AnalysisPlant Physiology, 2001
- Iron acquisition by plantsCurrent Opinion in Plant Biology, 1999
- Early Iron Deficiency Stress Response in Leaves of Sugar BeetPlant Physiology, 1995
- Reduction in phosphoribulokinase activity by antisense RNA in transgenic tobacco: effect on CO2 assimilation and growth in low irradianceThe Plant Journal, 1995
- Nucleotide Sequence of a cDNA Encoding a Heat-Shock Protein (HSP70) from Barley (Hordeum vulgare L.)Plant Physiology, 1994
- [34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranesPublished by Elsevier BV ,1987