1H NMR-based metabolite profiling as a potential selection tool for breeding passive resistance against Fusarium head blight (FHB) in wheat
- 1 July 2007
- journal article
- Published by Wiley in Molecular Plant Pathology
- Vol. 8 (4), 401-410
- https://doi.org/10.1111/j.1364-3703.2007.00400.x
Abstract
(1)H NMR measurements on protein-free extracts from wheat leaf and stem tissue were used to investigate the biochemical correlates of partial resistance to fungal species implicated in the Fusarium head blight (FHB) disease complex. The wheat genotypes included five commercial wheat cultivars and 116 wheat genotypes, from the CIMMYT international FHB breeding programme in Mexico, that had been bred for FHB disease resistance, utilizing exotic, typically Chinese, resistance sources. Principal component analysis of the nuclear magnetic resonance (NMR)-derived metabolite profiles revealed distinct separation of the commercial wheat cultivars from the majority of the CIMMYT wheat genotypes with the commercial cultivars exhibiting markedly higher carbohydrate concentrations. A cross-validated partial least squares (PLS) regression model of the metabolite profile against the partial disease resistance component latent period (delay in sporulation of the fungus on the plant tissue) predicted latent periods that were significantly correlated with the experimentally determined values (R(2) = 0.34, P < 0.001). Identified metabolites that were found in plants with shorter latent periods (higher disease susceptibility) included choline, the single most influential metabolite in the PLS model, betaine, the amino acids glutamine, glutamate and alanine, trans-aconitate and sucrose. Metabolites related to increased disease resistance included glucose and unassigned resonances in the carbohydrate and aromatic regions of the NMR spectra. The current study has demonstrated the potential of metabolite profiling as a tool for marker-assisted selection in commercial breeding for resistance to FHB in wheat.Keywords
This publication has 31 references indexed in Scilit:
- Components of resistance to fusarium head blight (FHB) in wheat detected in a seed-germination assay with Microdochium majus and the relationship to FHB disease development and mycotoxin accumulation from Fusarium graminearum infectionPlant Pathology, 2007
- Comparison of commercial European wheat cultivars to Fusarium infection of head and seedling tissuePlant Pathology, 2006
- Components of Pink Snow Mould Resistance in Winter Wheat are Expressed Prior to Cold Hardening and in Detached LeavesJournal of Phytopathology, 2006
- Impact of Fusarium culmorum on the Polysaccharides of Wheat FlourJournal of Agricultural and Food Chemistry, 2005
- Resistance of wheat to Fusarium spp. in an in vitro seed germination assay and preliminary investigations into the relationship with Fusarium head blight resistanceEuphytica, 2005
- Metabolite Profiling Using 1H NMR Spectroscopy for Quality Assessment of Green Tea, Camellia sinensis (L.)Journal of Agricultural and Food Chemistry, 2004
- Seeding Date Alters Carbohydrate Accumulation in Winter WheatCrop Science, 2001
- Metabolite profiling by one- and two-dimensional NMR analysis of complex mixturesProgress in Nuclear Magnetic Resonance Spectroscopy, 1996
- Alanine is a precursor in the biosynthesis of fumonisin B1 byFusarium moniliformeMycopathologia, 1993
- The isolation and identification of choline and betaine as the two major components in anthers and wheat germ that stimulate Fusarium graminearum in vitroPhysiological Plant Pathology, 1974