Detection of Somatic DNA Recombination in the Transgenic Mouse Brain
- 4 October 1991
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 254 (5028), 81-86
- https://doi.org/10.1126/science.1925563
Abstract
A DNA construct containing the bacterial beta-galactosidase gene (lacZ) was used to study somatic DNA recombination in the transgenic mouse brain. Recombination-positive areas of the adult brain were stained blue with X-gal, a substrate of beta-galactosidase. Blue-colored cells appeared soon after birth, and continued to emerge in postnatal tissue. Staining was prominent in sensory as opposed to motor regions of the brain, and was present in more than 70 discrete areas of the nervous system. The possibility of DNA rearrangement is discussed with respect to the development of the central nervous system.Keywords
This publication has 22 references indexed in Scilit:
- The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous systemCell, 1991
- The V(D)J recombination activating gene, RAG-1Cell, 1989
- A cytoarchitectonic atlas of the medial geniculate body of the opossum, Didelphys virginiana, with a comment on the posterior intralaminar nuclei of the thalamusJournal of Comparative Neurology, 1988
- Two Pairs of Recombination Signals Are Sufficient to Cause Immunoglobulin V-(D)-J JoiningScience, 1987
- The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brainCell, 1986
- Immunoglobulin gene transcription is activated by downstream sequence elementsCell, 1983
- Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genesNature, 1981
- Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genesNature, 1980
- An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JHCell, 1980
- Sequences at the somatic recombination sites of immunoglobulin light-chain genesNature, 1979