Multiangle Light Scattering: The Basic Tool for Macromolecular Characterization

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Abstract
In recent years, instrumentation capable of making multiangle light scattering (MALS) measurements have become an essential part of the chromatographer's armamentarium. Such instruments permit the determination of molar mass and size without assumptions or requirements for calibrated standards. As the number of scattering angles increase, there is a corresponding increase in the precision of the results calculated. The versatility of the MALS detection method permits application to a broad range of samples and separation techniques.