Relationship between arterial diameter and perfused tissue volume in myocardial microcirculation: a micro-CT-based analysis
Open Access
- 1 June 2004
- journal article
- Published by American Physiological Society in American Journal of Physiology-Heart and Circulatory Physiology
- Vol. 286 (6), H2386-H2392
- https://doi.org/10.1152/ajpheart.00682.2003
Abstract
The volume of myocardial tissue that is perfused by an epicardial coronary artery has been shown to be predictably related to the diameter of the epicardial arterial lumen. However, to what extent the intramyocardial microvasculature follows the epicardial rules remains unclear. To explore the relationship between the diameter of coronary arterioles and their subsequent perfused myocardial volumes, we quantified the volume of nonperfused myocardium resulting from an embolized arteriole of a certain diameter. We injected a single dose of microspheres selected from one of nine possible microsphere combinations (10, 30, and 100 μm diameter, each at three possible doses) into the left anterior descending coronary and/or left circumflex arteries of seven anesthetized pigs. At postmortem, the coronary arteries were infused with a radiopaque silicon polymer. Embolized myocardium (1 cm3) was scanned with a microcomputerized tomography scanner and resulted in three-dimensional images that consisted of 20 μm/side cubic voxels and a subvolume of the specimen with 4 μm/side cubic voxels. Image analysis provided the number and volumes of myocardial perfusion defects for each size and dose of microspheres. The smallest individual myocardial perfusion defects, which correspond to the volume of myocardium perfused by a single embolized arteriole, were found to be 0.0004 ± 0.0002, 0.02 ± 0.004, and 0.62 ± 0.099 mm3 for the 10-, 30-, and 100-μm microspheres, respectively. The number of myocardial perfusion defects in the embolized myocardium was inversely related to the dose of the injected microspheres. This reflects a clustering behavior that is consistent with a random distribution process of the individual embolized perfusion defects.Keywords
This publication has 21 references indexed in Scilit:
- Functional anatomy and hemodynamic characteristics of vasa vasorum in the walls of porcine coronary arteriesThe Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology, 2003
- Coronary Microembolization.Circulation Journal, 2003
- Transmural Distribution of Capillary Morphology as a Function of Coronary Perfusion Pressure in the Resting Canine HeartMicrovascular Research, 1995
- A quantitative study of the anatomy and distribution of coronary arteries in swine in comparison with other animals and manCardiovascular Research, 1986
- Regional myocardial volume perfused by the coronary artery branch: estimation in vivo.Circulation, 1986
- Does Visual Interpretation of the Coronary Arteriogram Predict the Physiologic Importance of a Coronary Stenosis?New England Journal of Medicine, 1984
- Coronary collateral circulation in the pig: correlation of collateral flow with coronary bed sizeBasic Research in Cardiology, 1981
- Quantitative study on the size of coronary artery supplying areas postmortemAmerican Heart Journal, 1977
- Die Verteilungsmuster der Koronararterien (Versorgungstypen) des menschlichen HerzensDeutsche Medizinische Wochenschrift (1946), 1976
- Direct measurement of intercapillary distance in beating rat heart in situ under various conditions of O2 supplyMicrovascular Research, 1969