A green capillary zone electrophoresis method for the simultaneous determination of piperacillin, tazobactam and cefepime in pharmaceutical formulations and human plasma
- 8 June 2015
- journal article
- research article
- Published by Wiley in Biomedical Chromatography
- Vol. 29 (12), 1811-1818
- https://doi.org/10.1002/bmc.3500
Abstract
A green, novel, rapid, accurate and reliable capillary zone electrophoresis method was developed and validated for the simultaneous determination of piperacillin, tazobactam and cefepime in pharmaceutical preparations. Separation was carried out using fused silica capillary (50 µm i.d. × 48.6 cm and 40.2 cm detection length) and applied potential of 20 kV (positive polarity) and a running buffer containing 15 m m sodium borate buffer adjusted to pH 9.3 with UV detection at 215 nm. Amoxicillin was used as an internal standard. The method was suitably validated according to International Conference on Harmonization guidelines. The method showed good linearity in the ranges of 10–100, 20–400 and 10–400 µg/mL with limits of quantitation of 1.87, 3.17 and 6.97 µg/mL and limits of detection of 0.56, 0.95 and 2.09 µg/mL for tazobactam, piperacillin and cefepime, respectively. The proposed method was successfully applied for the analysis of these drugs in their synthetic mixtures and co‐formulated injection vials. The method was extended to the in vitro determination of the two drugs in spiked human plasma. It is considered a ‘green’ method as it consumes no organic solvents. Copyright © 2015 John Wiley & Sons, Ltd.Keywords
Funding Information
- Deanship of Scientific Research (DSR), King Abdulaziz University, Jeddah, Saudi Arabia (363-007-D1433)
This publication has 26 references indexed in Scilit:
- Development and validation of a fast and uniform approach to quantify β-lactam antibiotics in human plasma by solid phase extraction-liquid chromatography–electrospray-tandem mass spectrometryTalanta, 2013
- High performance liquid chromatographic determination of plasma free and total tazobactam and piperacillinJournal of Chromatography B, 2009
- Orthodox and unorthodox clavulanate combinations against extended-spectrum β-lactamase producersClinical Microbiology & Infection, 2008
- An improved high-performance liquid chromatographic method with a solid-phase extraction for the determination of piperacillin and tazobactam: application to pharmacokinetic study of different dosage in Chinese healthy volunteersBiomedical Chromatography, 2007
- Quantitation and stability of piperacillin and tazobactam in plasma and ultrafiltrate from patients undergoing continuous venovenous hemofiltration by HPLCBiomedical Chromatography, 2005
- Microbial Resistance: Bacteria and MoreClinical Infectious Diseases, 2003
- Inadequate Antimicrobial Treatment: An Important Determinant of Outcome for Hospitalized PatientsClinical Infectious Diseases, 2000
- Cefepime Clinical PharmacokineticsClinical Pharmacokinetics, 1993
- PenicillinsDrugs, 1993
- Stability kinetics of piperacillin in aqueous solutionsInternational Journal of Pharmaceutics, 1982