Identification and Characterization of Human VCY2-Interacting Protein: VCY2IP-1, a Microtubule-Associated Protein-Like Protein
- 1 March 2004
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 70 (3), 775-784
- https://doi.org/10.1095/biolreprod.103.018531
Abstract
VCY2 is a testis-specific protein that locates in a frequently deleted azoospermia factor c region on chromosome Yq. Although its genomic structure has been characterized, the function of VCY2 is still unknown. To gain insight regarding the likely function of VCY2, we investigated the proteins that interact with VCY2 using the yeast two-hybrid system. We identified a novel VCY2 interaction partner, named VCY2IP-1, that encodes an open reading frame of 1059 amino acids. The amino acid sequence of VCY2IP-1 shows 59.3% and 41.9% homology to two human microtubule-associated proteins (MAPs), MAP1B and MAP1A, respectively. VCY2IP-1 has an extensive homology to the N-terminus and C-terminus regions of MAP1B and MAP1A, placing it within a large family of MAPs. We mapped VCY2IP-1 to chromosome 19p13.11. The VCY2IP-1 gene spans 15 kilobases (kb) and consists of seven exons. Northern blot analysis identified a single, intense band of approximately 3.2-kb VCY2IP-1 transcript, predominantly expressed in human testis. In situ hybridization of human testicular sections showed the localization of VCY2IP-1 transcripts in germ cells, and reverse transcription-polymerase chain reaction analysis demonstrated the presence of VCY2 and VCY2IP-1 transcripts in human ejaculated spermatozoa. Our expression data support the involvement of VCY2 and VCY2IP-1 in spermatogenesis. Based on the high homology of VCY2IP-1 with MAPs, we propose the involvement of VCY2 in the cytoskeletal network via interaction with VCY2IP-1.Keywords
This publication has 26 references indexed in Scilit:
- Genomic Organization, Physical Mapping, and Involvement in Yq Microdeletions of the VCY2 (BPY 2) GeneGenomics, 2001
- Prediction of complete gene structures in human genomic DNAJournal of Molecular Biology, 1997
- Characterization of microtubule-associated protein 1-associated protein kinases from rat brainNeurochemistry International, 1996
- Dephosphorylated but not phosphorylated microtubule associated protein MAP1B binds to microfilamentsFEBS Letters, 1996
- Microtubule-associated protein-2 in the rat testis: a novel site of expressionBiology of Reproduction, 1996
- Characterization of Microtubule-Associated Protein MAP1B: Phosphorylation State, Light Chains, and Binding to MicrotubulesBiochemistry, 1996
- Tau, the Neuronal Heat-Stable Microtubule-Associated Protein, is also Present in the Cross-Linked Microtubule Network of the Testicular Spermatid Manchette1Biology of Reproduction, 1992
- MAP1B is encoded as a polyprotein that is processed to form a complex N-terminal microtubule-binding domainNeuron, 1991
- Molecular Cloning of Microtubule‐Associated Protein 1 (MAP1A) and Microtubule‐Associated Protein 5 (MAP1B): Identification of Distinct Genes and Their Differential Expression in Developing BrainJournal of Neurochemistry, 1990
- MAP 4: a microtubule-associated protein specific for a subset of tissue microtubules.The Journal of cell biology, 1984