Role of Nucleolin in Human Parainfluenza Virus Type 3 Infection of Human Lung Epithelial Cells
Open Access
- 1 August 2004
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 78 (15), 8146-8158
- https://doi.org/10.1128/jvi.78.15.8146-8158.2004
Abstract
Human parainfluenza virus type 3 (HPIV-3) is an airborne pathogen that infects human lung epithelial cells from the apical (luminal) plasma membrane domain. In the present study, we have identified cell surface-expressed nucleolin as a cellular cofactor required for the efficient cellular entry of HPIV-3 into human lung epithelial A549 cells. Nucleolin was enriched on the apical cell surface domain of A549 cells, and HPIV-3 interacted with nucleolin during entry. The importance of nucleolin during HPIV-3 replication was borne out by the observation that HPIV-3 replication was significantly inhibited following (i) pretreatment of cells with antinucleolin antibodies and (ii) preincubation of HPIV-3 with purified nucleolin prior to its addition to the cells. Moreover, HPIV-3 cellular internalization and attachment assays performed in the presence of antinucleolin antibodies and purified nucleolin revealed the requirement of nucleolin during HPIV-3 internalization but not during attachment. Thus, these results suggest that nucleolin expressed on the surfaces of human lung epithelial A549 cells plays an important role during HPIV-3 cellular entry.Keywords
This publication has 60 references indexed in Scilit:
- Requirement for cyclophilin A for the replication of vesicular stomatitis virus New Jersey serotypeJournal of General Virology, 2003
- α4β1 Integrin Acts as a Cell Receptor for Murine Polyomavirus at the Postattachment LevelJournal of Virology, 2003
- Nuclear Targeting by the Growth Factor MidkineMolecular and Cellular Biology, 2002
- Contribution of the Human Parainfluenza Virus Type 3 HN-Receptor Interaction to Pathogenesis In VivoJournal of Virology, 2001
- Human Parainfluenza Virus Type 3 HN-Receptor Interaction: Effect of 4-Guanidino-Neu5Ac2en on a Neuraminidase-Deficient VariantJournal of Virology, 2001
- Interferon Action against Human Parainfluenza Virus Type 3: Involvement of a Novel Antiviral Pathway in the Inhibition of TranscriptionJournal of Virology, 2001
- CHEMOKINE RECEPTORS AS HIV-1 CORECEPTORS: Roles in Viral Entry, Tropism, and DiseaseAnnual Review of Immunology, 1999
- Glycophorin as a Receptor for Sendai VirusBiochemistry, 1996
- The Role of the Ganglioside GD1a as a Receptor for Sendai VirusBiochemistry, 1995
- A protein partially expressed on the surface of HepG2 cells that binds lipoproteins specifically is nucleolinBiochemistry, 1990