Early Cell Death Detection with Digital Holographic Microscopy
Open Access
- 31 January 2012
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 7 (1), e30912
- https://doi.org/10.1371/journal.pone.0030912
Abstract
Digital holography provides a non-invasive measurement of the quantitative phase shifts induced by cells in culture, which can be related to cell volume changes. It has been shown previously that regulation of cell volume, in particular as it relates to ionic homeostasis, is crucially involved in the activation/inactivation of the cell death processes. We thus present here an application of digital holographic microscopy (DHM) dedicated to early and label-free detection of cell death. We provide quantitative measurements of phase signal obtained on mouse cortical neurons, and caused by early neuronal cell volume regulation triggered by excitotoxic concentrations of L-glutamate. We show that the efficiency of this early regulation of cell volume detected by DHM, is correlated with the occurrence of subsequent neuronal death assessed with the widely accepted trypan blue method for detection of cell viability. The determination of the phase signal by DHM provides a simple and rapid optical method for the early detection of cell death.Keywords
This publication has 36 references indexed in Scilit:
- Digital holographic microscopy in human sperm imagingJournal of Assisted Reproduction and Genetics, 2011
- Cell morphology and intracellular ionic homeostasis explored with a multimodal approach combining epifluorescence and digital holographic microscopyJournal of Biophotonics, 2010
- Digital holographic microscopy real-time monitoring of cytoarchitectural alterations during simulated microgravityJournal of Biomedical Optics, 2010
- Major cell death pathways at a glanceMicrobes and Infection, 2009
- Physiology of Cell Volume Regulation in VertebratesPhysiological Reviews, 2009
- Apoptosis and necrosis: Detection, discrimination and phagocytosisMethods, 2008
- Noninvasive time-dependent cytometry monitoring by digital holographyJournal of Biomedical Optics, 2007
- Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopyOptics Express, 2005
- Supravital exposure to propidium iodide identifies apoptotic cells in the absence of nucleosomal DNA fragmentationCytometry, 1996
- An improved method to determine cell viability by simultaneous staining with fluorescein diacetate-propidium iodide.Journal of Histochemistry & Cytochemistry, 1985