Xylem Development and Cell Wall Changes of Soybean Seedlings Grown in Space
Open Access
- 5 February 2008
- journal article
- research article
- Published by Oxford University Press (OUP) in Annals of Botany
- Vol. 101 (5), 661-669
- https://doi.org/10.1093/aob/mcn001
Abstract
Plants growing in altered gravity conditions encounter changes in vascular development and cell wall deposition. The aim of this study was to investigate xylem anatomy and arrangement of cellulose microfibrils in vessel walls of different organs of soybean seedlings grown in Space. Seeds germinated and seedlings grew for 5 d in Space during the Foton-M2 mission. The environmental conditions, other than gravity, of the ground control repeated those experienced in orbit. The seedlings developed in space were compared with those of the control test on the basis of numerous anatomical and ultrastructural parameters such as number of veins, size and shape of vessel lumens, thickness of cell walls and deposition of cellulose microfibrils. Observations made with light, fluorescence and transmission electron microscopy, together with the quantification of the structural features through digital image analysis, showed that the alterations due to microgravity do not occur at the same level in the various organs of soybean seedlings. The modifications induced by microgravity or by the indirect effect of space-flight conditions, became conspicuous only in developing vessels at the ultrastructural level. The results suggested that the orientation of microfibrils and their assembly in developing vessels are perturbed by microgravity at the beginning of wall deposition, while they are still able to orient and arrange in thicker and ordered structures at later stages of secondary wall deposition. The process of proper cell-wall building, although not prevented, is perturbed in Space at the early stage of development. This would explain the almost unaltered anatomy of mature structures, accompanied by a slower growth observed in seedlings grown in Space than on Earth.Keywords
This publication has 46 references indexed in Scilit:
- Effects of hypergravity conditions on elongation growth and lignin formation in the inflorescence stem of Arabidopsis thalianaJournal of Plant Research, 2005
- Roles of microtubules and cellulose microfibril assembly in the localization of secondary-cell-wall deposition in developing tracheary elementsProtoplasma, 2004
- Auxin Is Required for Leaf Vein Pattern in ArabidopsisPlant Physiology, 1999
- Effects of hypergravity on growth and cell wall properties of cress hypocotylsJournal of Experimental Botany, 1996
- The effect of 8 days of microgravity on regeneration of intact plants from protoplastsPhysiologia Plantarum, 1994
- Gravity Effects on Cellulose AssemblyAmerican Journal of Botany, 1992
- The effect of exposure to microgravity on the development and structural organisation of plant protoplasts flown on Biokosmos 9Physiologia Plantarum, 1992
- Micromorphological and Ultrastructural Aspects of Spruce Wood Degradation by Wild-TypeSporotrichum pulverulentumand its Cellulase-less Mutant Cel 44Holzforschung, 1981
- Plant Microtechnique: Some Principles and New MethodsAmerican Journal of Botany, 1968
- Cytochimie ultrastructurale du nucléole — RNA et protéines intranucléolairesJournal of Ultrastructure Research, 1964