The structural basis of specific base-excision repair by uracil–DNA glycosylase
- 9 February 1995
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature
- Vol. 373 (6514), 487-493
- https://doi.org/10.1038/373487a0
Abstract
The 1.75-Å crystal structure of the uracil-DNA glycosylase from herpes simplex virus type-1 reveals a new fold, distantly related to dinucleotide-binding proteins. Complexes with a trideoxynucleotide, and with uracil, define the DNA-binding site and allow a detailed understanding of the exquisitely specific recognition of uracil in DNA. The overall structure suggests binding models for elongated single- and double-stranded DNA substrates. Conserved residues close to the uracil-binding site suggest a catalytic mechanism for hydrolytic base excision.Keywords
This publication has 22 references indexed in Scilit:
- The CCP4 suite: programs for protein crystallographyActa Crystallographica Section D-Biological Crystallography, 1994
- An algorithm for automatically generating protein topology cartoonsProtein Engineering, Design and Selection, 1994
- Crystallization and Preliminary X-ray Analysis of the Uracil-DNA Glycosylase DNA Repair Enzyme from Herpes Simplex Virus Type 1Journal of Molecular Biology, 1993
- PROCHECK: a program to check the stereochemical quality of protein structuresJournal of Applied Crystallography, 1993
- MOLSCRIPT: a program to produce both detailed and schematic plots of protein structuresJournal of Applied Crystallography, 1991
- Specificities and kinetics of uracil excision from uracil-containing DNA oligomers by Escherichia coli uracil DNA glycosylaseBiochemistry, 1991
- Improved methods for building protein models in electron density maps and the location of errors in these modelsActa Crystallographica Section A Foundations of Crystallography, 1991
- The Complete DNA Sequence of Varicella-Zoster VirusJournal of General Virology, 1986
- DNA sequence and expression of the B95-8 Epstein—Barr virus genomeNature, 1984
- Enzymic degradation of uracil-containing DNA. 3. Partial purification and characterization of a uracil DNA N-glycosidase from Bacillus subtilisBiochemistry, 1977