The Oxidation of Reduced Diphosphopyridine Nucleotide by an Ascorbate System from Cucumber.
Open Access
- 1 May 1954
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 29 (3), 265-269
- https://doi.org/10.1104/pp.29.3.265
Abstract
Crude extracts from cucumber were shown to induce a rapid, cyanide sensitive oxidation of diphosphopyridine nucleotide (DPNH) when ascorbic acid was added in small amounts. The oxidation system involves ascorbic oxidase present in the extracts and another component which is extremely sensitive to 2,3-dimer-captopropanol and p-chloromercuribenzoic acid. Dehydroascorbic acid did not function as H-acceptor and it appears that some other product of the enzymic oxidation of ascorbic acid is the H-acceptor when DPNH is being oxidized. The ascorbic-induced DPNH oxidation has been linked successfully in spectrophotometric experiments with alcohol and its dehydrogenase, and the system thus provides a chain from substrate to atmospheric O2.This publication has 8 references indexed in Scilit:
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