Expression of acylamidase gene in Rhodococcus erythropolis strains
- 23 September 2014
- journal article
- Published by Pleiades Publishing Ltd in Russian Journal of Genetics
- Vol. 50 (9), 1003-1007
- https://doi.org/10.1134/s1022795414090087
Abstract
The expression of a new acylamidase gene from R. erythropolis TA37 was studied in Rhodococcus erythropolis strains. This acylamidase, as a result of its unique substrate specificity, can hydrolyse N-substituted amides (4′-nitroacetanilide, N-isopropylacrylamide, N′N-dimethylaminopropylacrylamide). A new expression system based on the use of the promoter region of nitrile hydratase genes from R. rhodochrous M8 was created to achieve constitutive synthesis of acylamidase in R. erythropolis cells. A fourfold improvement in the acylamidase activity of recombinant R. erythropolis cells as compared with the parent wild-type strain was obtained through the use of the new expression system.Keywords
This publication has 22 references indexed in Scilit:
- Analyses of both the alkB Gene Transcriptional Start Site and alkB Promoter-Inducing Properties of Rhodococcus sp. Strain BCP1 Grown on n -AlkanesApplied and Environmental Microbiology, 2011
- The Pfam protein families databaseNucleic Acids Research, 2009
- Cloning and nucleotide sequence of carbaryl hydrolase gene (cahA) from Arthrobacter sp. RC100Journal of Bioscience and Bioengineering, 2006
- Purification and Characterization of Allophanate Hydrolase (AtzF) from Pseudomonas sp. Strain ADPJournal of Bacteriology, 2005
- Enzymatic hydrolysis of cyanohydrins with recombinant nitrile hydratase and amidase from hodococcus erythropolisBiotechnology Letters, 2004
- A novel R‐stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine‐2‐tert‐butylcarboxamideJBIC Journal of Biological Inorganic Chemistry, 2004
- Cloning and Heterologous Expression of an Enantioselective Amidase from Rhodococcus erythropolis Strain MP50Applied and Environmental Microbiology, 2002
- Transcriptional analysis of the nitrile‐degrading operon from Rhodococcus sp. ACV2 and high level production of recombinant amidase with an Escherichia coli – T7 expression systemJournal of Applied Microbiology, 1999
- Plasmid transfer by conjugation fromEscherichia colito Gram-positive bacteriaFEMS Microbiology Letters, 1987
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970