Characterisation of CYP2C8, CYP2C9 and CYP2C19 polymorphisms in a Ghanaian population
Open Access
- 2 December 2009
- journal article
- Published by Springer Science and Business Media LLC in BMC Medical Genetics
- Vol. 10 (1), 124
- https://doi.org/10.1186/1471-2350-10-124
Abstract
Genetic influences on drug efficacy and tolerability are now widely known. Pharmacogenetics has thus become an expanding field with great potential for improving drug efficacy and reducing toxicity. Many pharmacologically-relevant polymorphisms do show variability among different populations. Knowledge of allelic frequency distribution within specified populations can be useful in explaining therapeutic failures, identifying potential risk groups for adverse drug reactions (ADRs) and optimising doses for therapeutic efficacy. We sought to determine the prevalence of clinically relevant Cytochrome P450 (CYP) 2C8, CYP2C9, and CYP2C19 variants in Ghanaians. We compared the data with other ethnic groups and further investigated intra country differences within the Ghanaian population to determine its value to pharmacogenetics studies. RFLP assays were used to genotype CYP2C8 (*2, *3, *4) variant alleles in 204 unrelated Ghanaians. CYP2C9*2 and CYP2C19 (*2 and *3) variants were determined by single-tube tetra-primer assays while CYP2C9 (*3, *4, *5 and *11) variants were assessed by direct sequencing. Allelic frequencies were obtained for CYP2C8*2 (17%), CYP2C8*3 (0%), CYP2C8*4 (0%), CYP2C9*2 (0%), CYP2C9*3 (0%), CYP2C9*4 (0%), CYP2C9*5 (0%), CYP2C9*11 (2%), CYP2C19*2 (6%) and CYP2C19*3 (0%). Allele frequency distributions for CYP2C8, CYP2C9 and CYP2C19 among the Ghanaian population are comparable to other African ethnic groups but significantly differ from Caucasian and Asian populations. Variant allele frequencies for CYP2C9 and CYP2C19 are reported for the first time among indigenous Ghanaian population.Keywords
This publication has 41 references indexed in Scilit:
- Short communication: High prevalence of the cytochrome P450 2C8*2 mutation in Northern GhanaTropical Medicine & International Health, 2005
- Genetic predisposition to acute gastrointestinal bleeding after NSAIDs useBritish Journal of Pharmacology, 2004
- Allelic variants of cytochromes P450 2C modify the risk for acute myocardial infarctionPharmacogenetics, 2003
- Genetic polymorphisms ofCYP2C9andCYP2C19in the Beninese and Belgian populationsBritish Journal of Clinical Pharmacology, 2003
- Allele and genotype frequency of CYP2C19 in a Tamilian populationBritish Journal of Clinical Pharmacology, 2003
- Clinical relevance of genetic polymorphisms in the human CYP2C subfamilyBritish Journal of Clinical Pharmacology, 2001
- Genetic polymorphism of cytochrome P450 2C9 in a Caucasian and a black African populationBritish Journal of Clinical Pharmacology, 2001
- Cytochrome P4502C9: an enzyme of major importance in human drug metabolismBritish Journal of Clinical Pharmacology, 1998
- Frequencies of the defective CYP2C19 alleles responsible for the mephenytoin poor metabolizer phenotype in various Oriental, Caucasian, Saudi Arabian and American black populationsPharmacogenetics, 1997
- Biochemistry and molecular biology of the human CYP2C subfamilyPharmacogenetics, 1994